Biotin anti-mouse CCL11 (Eotaxin) Antibody

Pricing & Availability
Clone
Poly5222 (See other available formats)
Other Names
Eotaxin, Eotaxin-1, small inducible cytokine subfamily A, member 11 (SCYA11)
Isotype
Goat Polyclonal Ig
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Cat # Size Price Quantity Avail. Save
522204 50 µg 225 CHF
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Description

CCL11, also known as Eotaxin, is a small cytokine belonging to the CC chemokine family. The full length of CCL11 is 97 amino acids (a.a.) with a 23 a.a. signal peptide, which is cleaved to generate a 74 a.a. mature protein. The molecular mass of CCL11 is about 10 kD, and its gene is located on the q arm of chromosome 17. CCL11 expression has been found in normal tissues such as smooth muscle and small intestine. Endothelial cells, smooth muscle cells, epithelial cells, alveolar macrophages, and eosinophils are known to produce CCL11. Mouse CCL11 shares a 61% and 96% amino acid sequence identity to both human and rat, respectively.

CCL11 is produced when inflammatory cytokines, such as IL-5 and TNF-α, stimulate lung endothelial cells, fibroblasts, and smooth muscle cells in response to allergens. As a potent eosinophil chemoattractant, via binding to its major receptor, CCR3, CCL11 efficiently induces the accumulation of eosinophils, a response associated with allergic inflammation. Therefore, atopic dermatitis, asthma, and parasitic infections are a few of the eosinophilic inflammatory diseases directly related to CCL11. In addition, CCL11 induces the formation of blood vessels and other angiogenic responses which are accompanied by inflammatory infiltration; it has been shown to regulate tumor development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.5 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of CCL11 recombinant protein ranging from 500 to 7.81 pg/mL is recommended for each ELISA plate.

It is recommended that the reagent be titrated for optimal performance.

Application Notes

ELISA Detection: The biotinylated Poly5222 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified L403H11 (Cat. No. 683202) antibody as the capture antibody.

 

Note: For testing mouse CCL11 in cell culture supernatant, serum, plasma and other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 443907 & 443908) are specially developed and recommended.

RRID
AB_2686954 (BioLegend Cat. No. 522204)

Antigen Details

Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
20292 View all products for this Gene ID
UniProt
View information about CCL11 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 04.03.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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