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Biotin anti-mouse 4-1BB Ligand (CD137L) Antibody

Pricing & Availability
Clone
TKS-1 (See other available formats)
Regulatory Status
RUO
Other Names
4-1BBL, CD137L, TNFSF9, 4-1BB Ligand
Isotype
Rat IgG2a, κ
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Product Citations
publications
TKS-1_Biotin_072407
Mouse B cell line 2PK3 stained with biotinylated TKS-1, followed by Sav-PE
  • TKS-1_Biotin_072407
    Mouse B cell line 2PK3 stained with biotinylated TKS-1, followed by Sav-PE
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107103 50 µg 83 CHF
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Description

4-1BB ligand, also known as CD137L, is a 97 kD member of the TNF superfamily present on antigen-presenting cells (dendritic cells and macrophages) and activated B and T lymphocytes. 4-1BBL interacts with 4-1BB (CD137) to induce T and B cell costimulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse B lymphoma line 2PK-3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The TKS-1 antibody inhibits binding of soluble 4-1BB to 4-1BBL in vitro. Additional reported applications (for the relevant formats) include: in vitro blocking the binding of 4-1BB to 4-1BBL1. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107106) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107104) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 107108).

Application References

(PubMed link indicates BioLegend citation)
  1. Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block)
  2. Futagawa T, et al. 2002. Int. Immunol. 14:275.
  3. Nishimoto H, et al. 2005. Blood 106:4241. (FC) PubMed
  4. De Keersmaecker B, et al. 2011. J Leukoc Biol. 89:989. PubMed.
  5. Zhao Y, et al. 2012. Immuno Lett. 141:220. PubMed.
Product Citations
  1. Wan D, et al. 2021. Exp Mol Med. 53:654. PubMed
  2. Zhao J, Croft M 2012. Immunol Lett. 141:220. PubMed
  3. Keersmaecker B, et al. 2011. J Leukoc Biol. 89:989. PubMed
RRID
AB_313304 (BioLegend Cat. No. 107103)

Antigen Details

Structure
NGF/TNF superfamily, 97 kD
Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function
T cell costimulation
Ligand/Receptor
4-1BB (CDw137)
Cell Type
B cells, Dendritic cells, Macrophages, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Pollok KE, et al. 1995. Eur. J. Immunol. 25:488.
2. DeBenedette MA, et al. 1997. J. Immunol. 158:551.
3. Pollok KE, et al. 1993. J. Immunol. 150:771.
4. Pollok KE, et al. 1994. Eur. J. Immunol. 24:367.
5. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.

Gene ID
21942 View all products for this Gene ID
UniProt
View information about CD137L on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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