- Clone
- IA6-2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ig delta chain C region
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Human peripheral blood lymphocytes were stained with CD19 (clone HIB19) APC and biotinylated anti-human IgD (clone IA6-2, top) or mouse IgG1, κ isotype control (bottom), followed by Sav-PE. -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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348211 | 50 µg | 71 CHF | ||||
348212 | 500 µg | 167 CHF |
IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IgD
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections4 and spatial biology (IBEX)5,6.
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Application References
(PubMed link indicates BioLegend citation) -
- Chen K, et al. 2009. Nat. Immunol. 10:889.
- Lee CH, et al. 2005. J. Exp. Med. 203:63.
- Sutter JA, et al. 2008. Clin. Immunol. 126:282.
- Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
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AB_10680788 (BioLegend Cat. No. 348211)
AB_10681720 (BioLegend Cat. No. 348212)
Antigen Details
- Structure
- Exists in a transmembranal and a soluble form
- Distribution
-
Naïve B cells
- Function
- Antigen binding, B cell activation
- Interaction
- The CD79a/CD79b heterodimer
- Cell Type
- B cells
- Biology Area
- Immunology
- Antigen References
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1. Geisberger R, et al. 2006. Immunology 118:429.
2. Weller S, et al. 2005. Eur. J. Immunol. 35:2789.
3. Brandtzaeg P and Johansen FE. 2005. Immunol. Rev. 206:32. - Gene ID
- 3495 View all products for this Gene ID
- UniProt
- View information about IgD on UniProt.org
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 647 anti-human IgD
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PerCP anti-human IgD
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Brilliant Violet 605™ anti-human IgD
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Alexa Fluor® 700 anti-human IgD
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Purified anti-human IgD
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PE anti-human IgD
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Biotin anti-human IgD
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FITC anti-human IgD
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PerCP/Cyanine5.5 anti-human IgD
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PE/Cyanine7 anti-human IgD
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Alexa Fluor® 488 anti-human IgD
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APC/Cyanine7 anti-human IgD
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Brilliant Violet 510™ anti-human IgD
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APC anti-human IgD
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Pacific Blue™ anti-human IgD
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Brilliant Violet 421™ anti-human IgD
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Purified anti-human IgD (Maxpar® Ready)
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PE/Dazzle™ 594 anti-human IgD
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APC/Fire™ 750 anti-human IgD
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Brilliant Violet 785™ anti-human IgD
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TotalSeq™-A0384 anti-human IgD
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TotalSeq™-C0384 anti-human IgD
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TotalSeq™-B0384 anti-human IgD
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PE/Cyanine5 anti-human IgD
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TotalSeq™-D0384 anti-human IgD
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