Biotin anti-human CD73 (Ecto-5'-nucleotidase) Antibody

Pricing & Availability
Clone
AD2 (See other available formats)
Workshop
V B-CD73.3
Other Names
Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_AD2_Biotin_CD73_Antibody_FC_1_121514
Human peripheral blood lymphocytes were stained CD3 APC and biotinylated CD73 (clone AD2) (top), or biotinylated mouse IgG1, κ isotype control (bottom), followed by Streptavidin PE.
  • A_AD2_Biotin_CD73_Antibody_FC_1_121514
    Human peripheral blood lymphocytes were stained CD3 APC and biotinylated CD73 (clone AD2) (top), or biotinylated mouse IgG1, κ isotype control (bottom), followed by Streptavidin PE.
  • B_AD2_Biotin_CD73_Antibody_FC_2_121514
Cat # Size Price Quantity Avail. Save
344017 50 µg 125 CHF
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Description

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include:immunofluorescence3.

clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1993. J. Immunol. 151:6933.
  2. Liao J, et al. 2011. J Endod. 37:1217. PubMed
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
RRID
AB_2564561 (BioLegend Cat. No. 344017)

Antigen Details

Structure
GPI-linked 5'-nucleotidase, 70 kD
Distribution

Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells

Function
Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen  S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.

Gene ID
4907 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 12.16.2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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