Biotin anti-human CD3 Antibody anti-CD3

Pricing & Availability

Clone: SK7 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ
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Product Citations: publications

SK7_biotin_093010 — Human peripheral blood lymphocytes stained with biotinylated anti-human CD3 (clone SK7, filled histogram) or biotinylated mouse IgG1, κ (open histogram) isotype control, followed by SAV-PE

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344820

100 µg

141 CHF

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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^4,5,8, immunofluorescent staining⁶, and Western blotting³.

Application References (PubMed link indicates BioLegend citation)

Kan EA, et al. 1983. J. Immunol. 131:536.
Wood GS, et al. 1985. Am. J. Pathol. 120:371.
Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
Carbone A, et al. 1999. Blood 93:2319. (IHC)
Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
Rutjens E, et al. 2007. J. Immunol. 178:1702.
Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

Product Citations

Comrie W, et al. 2015. J Cell Biol. 208:475. PubMed
Rossatti P, et al. 2019. Cells. 1.297222222. PubMed
Ye CJ, et al. 2018. Genome Res. 28:1812. PubMed

RRID

AB_10662538 (BioLegend Cat. No. 344820)

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, during thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD3 Reagents Request Custom Conjugation

Description	Clone	Applications
APC/Fire™ 750 anti-human CD3	SK7	FC
Biotin anti-human CD3	SK7	FC,ICC
Purified anti-human CD3	SK7	FC,ICC,IHC-F,WB
FITC anti-human CD3	SK7	FC
PE anti-human CD3	SK7	FC
Alexa Fluor® 488 anti-human CD3	SK7	FC
APC anti-human CD3	SK7	FC
PerCP/Cyanine5.5 anti-human CD3	SK7	FC
PerCP anti-human CD3	SK7	FC
PE/Cyanine7 anti-human CD3	SK7	FC
APC/Cyanine7 anti-human CD3	SK7	FC
Alexa Fluor® 700 anti-human CD3	SK7	FC
Pacific Blue™ anti-human CD3	SK7	FC
Alexa Fluor® 647 anti-human CD3	SK7	FC
Brilliant Violet 510™ anti-human CD3	SK7	FC
Brilliant Violet 421™ anti-human CD3	SK7	FC
Brilliant Violet 605™ anti-human CD3	SK7	FC
Brilliant Violet 711™ anti-human CD3	SK7	FC
FITC anti-human CD3	SK7	FC
PE anti-human CD3	SK7	FC
Brilliant Violet 785™ anti-human CD3	SK7	FC
PE/Dazzle™ 594 anti-human CD3	SK7	FC
Brilliant Violet 750™ anti-human CD3	SK7	FC
TotalSeq™-A0049 anti-human CD3	SK7	PG
PerCP/Cyanine5.5 anti-human CD3	SK7	FC
TotalSeq™-C0049 anti-human CD3	SK7	PG
Spark Blue™ 550 anti-human CD3	SK7	FC
PE/Cyanine7 anti-human CD3	SK7	FC
TotalSeq™-B0049 anti-human CD3	SK7	PG
Alexa Fluor® 660 anti-human CD3	SK7	FC
APC/Fire™ 810 anti-human CD3	SK7	FC
Spark NIR™ 685 anti-human CD3	SK7	FC
PE/Fire™ 640 anti-human CD3	SK7	FC
PE/Fire™ 700 anti-human CD3	SK7	FC
APC anti-human CD3	SK7	FC
PerCP anti-human CD3	SK7	FC
APC/Fire™ 750 anti-human CD3	SK7	FC
GMP FITC anti-human CD3	SK7	FC
PE/Cyanine5 anti-human CD3 Antibody	SK7	FC
GMP PE anti-human CD3	SK7	FC
GMP APC anti-human CD3	SK7	FC
GMP PerCP/Cyanine5.5 anti-human CD3	SK7	FC
Spark YG™ 593 anti-human CD3	SK7	FC
GMP PerCP anti-human CD3	SK7	FC
Spark Violet™ 500 anti-human CD3	SK7	FC
Brilliant Violet 650™ anti-human CD3	SK7	FC
Spark PLUS UV™ 395 anti-human CD3	SK7	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 08.16.2019

For Research Use Only. Not for diagnostic or therapeutic use.

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