Biotin anti-human CD218a (IL-18Rα) Antibody

Pricing & Availability
Clone
H44 (See other available formats)
Workshop
HCDM listed
Other Names
IL-18 receptor alpha chain, IL-18 receptor 1, IL-18 receptor related protein, CDw218a
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
H44_Biotin_021508
Human peripheral blood lymphocytes stained with biotinylated H44, followed by Sav-PE
  • H44_Biotin_021508
    Human peripheral blood lymphocytes stained with biotinylated H44, followed by Sav-PE
Cat # Size Price Quantity Avail. Save
313806 100 µg 185 CHF
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Description

IL-18 receptor is composed of an α and a β subunit that combine to form a high affinity receptor for IL-18. IL-18 receptor α chain, also known as CDw218a, is a 75-80 kD type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of CDw218a on lymphocytes is upregulated after activation. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human NK cell line NK0 constitutively expressing IL-18 receptors
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

 The H44 antibody is specific for IL-18 receptor α chain. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and neutralization1.

Application References

(PubMed link indicates BioLegend citation)
  1. Kitasato Y, et al. 2004. Am. J. Respir. Cell Mol. Biol. 31:619. (IHC)
  2. Vermot-Desroches C, et al. 2005. Cell Immunol. 236:101. (FC)
RRID
AB_2123649 (BioLegend Cat. No. 313806)

Antigen Details

Structure
Type I membrane protein, IL-1 receptor family, approximately 75-80 kD
Distribution

Expressed on NK cells and neutrophils, IL-12 activated T and B cells, endothelial cells, and smooth muscle cells; highly expressed on Hodgkin's disease cell lines

Function
Binds IL-18 with low affinity; high affinity binding site formed with IL-18Rβ
Ligand/Receptor
IL-18
Cell Type
B cells, Endothelial cells, Neutrophils, NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Torigoe K, et al. 1997. J. Biol. Chem. 272:25737.
2. Gerdes N, et al. 2002. J. Exp. Med. 195:245.
3. Airoldi I, et al. 2000. J. Immunol. 165:6880.

Gene ID
8809 View all products for this Gene ID
UniProt
View information about CD218a on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 10.31.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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