Biotin anti-human CD172a/b (SIRPα/β) Antibody

Pricing & Availability
Clone
SE5A5 (See other available formats)
Other Names
SIRPa, SIRPb, SIRPalpha/beta, BIT, SHPS1, MFR, P84, PTPNS1, CD172 antigen-like family member A, CD172 antigen-like family member B
Isotype
Mouse IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
SE5A5_Biotin_020708
Human peripheral blood granulocytes stained with biotinylated SE5A5, followed by Sav-PE
  • SE5A5_Biotin_020708
    Human peripheral blood granulocytes stained with biotinylated SE5A5, followed by Sav-PE
Cat # Size Price Quantity Avail. Save
323804 100 µg 180 CHF
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Description

CD172a, also known as signal-regulatory protein α (SIRPα), src homology 2 domain-containing phosphatase substrate-1 (SHPS1), PTPNS1, BIT, MFR, and P84, is a 75-110 kD transmembrane glycoprotein involved in receptor tyrosine kinase coupled signaling pathway. It belongs to the Ig superfamily and is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion, and phagocytosis. CD47 (IAP) is the extracellular ligand for CD172a. SIRPα was recently demonstrated to be a specifc marker for cardiomyocytes derived from human pluripotent stem cells2.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3/hu-SIRPα cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone SE5A5 recognizes a common epitope on SIRPα (90 kD) and SIRPβ (50 kD)3. A high degree of homology has been found between SIRP family isoforms alpha and beta at the level of extracellular domains. Consequently, many anti SIRP antibody clones, such as SE5A5, have been reported to cross react with several SIRP isoforms1,4,5. It reacts with CD172a and has weak cross-reaction with CD172b. This antibody is able to block the binding of SIRPα (SIRPα1 and SIRPα2) to CD471,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Seiffert M, et al. 1999. Blood 94:3633.
  2. Dubois NC, et al. 2011. Nat. Biotechnol. 29:1011.
  3. Barros MM, et al. 2009. Transfusion 49:154.
  4. Liu Y, et al. 2005. J. Biol. Chem. 280:36132.
  5. Barclay AN. 2009. Curr. Opin. Immunol. 21:47.
  6. Florian S, et al. 2005. J. Leukoc. Biol. 77:984.
Product Citations
  1. Mittag D, et al. 2011. J Immunol. 186:6207. PubMed
Publication Library
RRID
AB_830703 (BioLegend Cat. No. 323804)

Antigen Details

Structure
75-110 kD glycoprotein , signal-regulatorey-protein family, Ig superfamily
Distribution

Monocytes/macrophages, granulocytes, dendritic cells, neurons, cardiomyocytes

Function
Negative regulation of leukocyte adhesion and transmigration, macrophage fusion, phagocytosis
Ligand/Receptor
CD47
Cell Type
Monocytes, Macrophages, Granulocytes, Dendritic cells, Neurons
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Seiffert M, et al. 1999. Blood 94:3633.
2. Seiffert M, et al. 2001. Blood 97:2741.
3. Timms JF, et al. 1998. Mol. Cell Biol. 18:3838.
4. Barclay AN and Brown MH. 2006. Nat. Rev. Immnuol. 6:457.

Gene ID
140885 View all products for this Gene ID
UniProt
View information about CD172a/b on UniProt.org

Reviews

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Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 10.25.2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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