Biotin anti-human C5a/C5a(desArg)/C5 Antibody

Pricing & Availability
Clone
G25/2 (See other available formats)
Other Names
C5AR1, C5A, C5AR
Isotype
Mouse IgG1, κ
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Cat # Size Price Quantity Avail. Save
518305 50 µg 95 CHF
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518306 500 µg 295 CHF
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Description

C5, complement component 5, is a protein involved in the complement system. It is cleaved into C5a and C5b by the C3/C5 convertase. The smaller fragment C5a is an anaphylatoxin and  is released into the fluid surroundings. C5a is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis). C5a contains 74 amino acids and is rapidly metabolised by a serum enzyme, carboxypeptidase B to a 73 amino acid form, C5a des-Arg. The major fragment C5b binds to the membrane initiating the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human C5a
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
WB, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of C5a recombinant protein ranging from 15.6 to 1000 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The G25/2 antibody can neutralize the bioactivity of natural or recombinant C5a.

Neutralization: The G25/2 antibody can neutralize the bioactivity of natural or recombinant C5a/C5a(desArg)/C5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human C5a/C5a(desArg)/C5 bioactivity in vivo and in vitro.

ELISA Detection: The biotinylated G25/2 antibody (Cat. No. 518306) is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified C17/5 antibody as the capture antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Oppermann M, et al. 1991. Kidney Int. 40:939947.
  2. Oppermann M, et al. 1992. Immunopharm. 24:119134.
  3. Elsner J, et al. 1994. Blood 83:3324.
RRID
AB_10900415 (BioLegend Cat. No. 518305)
AB_10896948 (BioLegend Cat. No. 518306)

Antigen Details

Biology Area
Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Lundwall AB, et al. 1985. J. Biol. Chem. 260:2108.
2. Fernandez HN, et al. 1978. J. Biol. Chem. 253:6955.
3. Tack BF, et al. 1979. Biochemistry 18:1490.

Gene ID
727 View all products for this Gene ID
UniProt
View information about C5a/C5adesArg/C5 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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