β/γ Secretase Antibody Sampler Kit

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1_B-Y_Secretase_Antibody_Sampler_kit_1_090618
IHC staining of anti-Nicastrin antibody (clone 9C3) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1_B-Y_Secretase_Antibody_Sampler_kit_1_090618
    IHC staining of anti-Nicastrin antibody (clone 9C3) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2_B-y_Secretase_Antibody_Sampler_kit_WB_1_090618
    Western blot of anti-Nicastrin antibody (clone 9C3) and isotype-matched control mouse IgG2b. Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.5 µg/mL of anti-Nicastrin or mouse IgG overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. In human the antibody also detects the deglycosylated nicastrin.
  • 3_B-y_Secretase_Antibody_Sampler_kit_WB_2_090618
    Western blot of purified anti-BACE1 antibody (clone A17035K). Lane 1: Molecular weight marker; Lane 2: 20 µg of total human brain lysate; Lane 3: 20 µg of human brain membrane lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 4_B-y_Secretase_Antibody_Sampler_kit_WB_3_090618
    Western blot of anti-Presenilin 2 antibody (clone PS2). Lane 1: Molecular weight marker; Lane 2: SH-SY5Y Nuclear Fraction. The blot was incubated with the primary antibody at 1 µg/mL overnight at 4°C, followed by incubation with a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection
  • 5_B-y_Secretase_Antibody_Sampler_kit_WB_4_090618
    Western blot of purified anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of Mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 1 µg/mL of TUJ1 or mouse IgG overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 6_B-Y_Secretase_Antibody_Sampler_kit_2_090618
    IHC staining of purified anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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899906 1 kit 370 CHF
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Description

The β/γ Secretase Antibody Sampler Kit offers flexibility for sampling and detection of β- and γ-secretase proteases that are essential components in the processing of amyloid precursor protein and generation of amyloid beta peptide.
 

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Format Clone Size Reactivity Isotype
Anti-Tubulin β 3 (TUBB3) Purified TUJ1 25 μl Human, Mouse, Rat Mouse IgG2a
Anti-Nicastrin Purified  9C3 25 μg Human, Mouse, Rat Mouse IgG2b, κ
 Anti-BACE1 Purified  A17035K 25 μg Human Mouse IgG2b, κ
 Anti-Presenilin 2 Purified PS2 25 μg Human Mouse IgG2a, κ
 Anti-Presenilin 1 (N terminus) Purified NT1 25 μl Human Mouse IgG1

* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Formulation
Please refer to individual product datasheets of the purified formats for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

IHC-P, WB - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by immunohistochemical staining on formalin-fixed paraffin-embedded tissue or Western blotting. For immunohistochemistry, the suggested uses of these reagents are as follows:

Anti-Tubulin β 3 (TUBB3) (Previously Covance catalog# MMS-435P): 1.0 - 5.0 µg/ml

For Western blotting, the suggested uses of these reagents are as follows:

Anti-Nicastrin: 0.1 - 0.5 µg/ml
Anti-BACE1: 1.0 - 5.0 µg/ml
Anti-Presenilin 2: 1.0 - 10 µg/ml
Anti-Presenilin 1 (N terminus) (Previously Covance catalog# SIG-39194): 10 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For validated or reported applications for these antibodies, please see individual product datasheets.

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Antigen References
  1. Yang D, et al. 2002. J. Biol. Chem. 31:28135.
  2. Johnson DS, et al. 2017. Cold Spring Harb Perspect Med. 10.1101/cshperspect.a024067.
  3. Zhang X, et al. 2014. Front Cell Neurosci. 10.3389/fncel.2014.00427.
  4. Vassar R, et al. 2014. J. Neurochem. 130:4-28.
  5. Kandalepas PC, Vassar R. 2014. Curr. Alzheimers Res. 11:441-9.
  6. Kandalepas PC, Vassar R. 2012. J. Neurochem. 120(Suppl. 1):55-61.
  7. Gruninger-Leitch F, et al. 2002. J. Biol. Chem. 277:4687-93.
  8. Koelsch G. 2017. Molecules. 22(10): E1723.
  9. Brunello L, et al. 2009. J. Cell Mol. Med. 13:3358. (WB) PubMed
  10. Hutton M, Hardy J. 1997. Hum. Mol. Genet. 6:1639.
  11. Mathews PM, et al. 2000. Brain expression of presenilins in sporadic and early-onset, familial alzheimer's disease. Mol. Med. 6(10):878-91.
  12. Cohen RM, et al. 2013. J. Neurosci. 15:6245. (WB) PubMed
Gene ID
23385 View all products for this Gene ID 23621 View all products for this Gene ID 5664 View all products for this Gene ID 10381 View all products for this Gene ID 5663 View all products for this Gene ID

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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