APC/Fire™ 750 anti-mouse CD205 (DEC-205) Antibody

Pricing & Availability
Clone
NLDC-145 (See other available formats)
Regulatory Status
RUO
Other Names
DEC-205
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
NLDC-145_APCFire750_CD205_Antibody_032019
C57BL/6 mouse bone marrow cells stained with Ly-6G/Ly-6C (clone RB6-8C5) Pacific Blue™ and CD205 (DEC-205) (clone NLDC-145) APC/Fire™ 750 (left) or Rat IgG2a, κ APC/Fire™ 750 isotype control (right).
  • NLDC-145_APCFire750_CD205_Antibody_032019
    C57BL/6 mouse bone marrow cells stained with Ly-6G/Ly-6C (clone RB6-8C5) Pacific Blue™ and CD205 (DEC-205) (clone NLDC-145) APC/Fire™ 750 (left) or Rat IgG2a, κ APC/Fire™ 750 isotype control (right).
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Check Availability Save
138219 25 µg 115,00 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
138220 100 µg 335,00 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD205, also known as DEC-205, is a 205 kD integral membrane protein homologous to the macrophage mannose receptor. It is a type I cell surface protein that belong to the C-type lectin family.  CD205 is expressed at high levels by dendritic cells and thymic epithelial cells.  It is also expressed by a number of other cell types, such as B lymphocytes, macrophages, Langerhans cells, bone marrow stromal cells, granulocytes, epithelial cells of pulmonary airways, and the capillaries of the brain.  CD205 is a novel endocytic receptor used by dendritic cells and thymic epithelial cells to direct captured antigens from the extracellular space to specialized antigen processing.  It mediates antigen uptake and presentation and cross-presentation to T cells.  It has been reported that CD205 acts as a recognition receptor for dying cells, potentially provides an important pathway for the uptake of self-antigen in the intrathymic environment, and is involved in peripheral tolerance.  Antibody-mediated antigen-targeting via the DEC-205 receptor increases the efficiency of vaccination for T cell immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse lymph node tissue
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1, Western Blot1-3, and immunoprecipitation of bone marrow dendritic cell extracts2.

Application References

(PubMed link indicates BioLegend citation)
  1. Witmer-Pack MD, et al. 1995. Cell. Immun. 163:157. (IHC, WB)
  2. Swiggard WJ, et al. 1995. Cell. Immun. 165:302. (WB, IP)
  3. Bonifaz LC, et al. 2004. J. Exp. Med. 199:815. (WB)
  4. Yamazaki S, et al. 2002. J. Immunol. 181:6923. (FC)
  5. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC)
RRID
AB_2800645 (BioLegend Cat. No. 138219)
AB_2800646 (BioLegend Cat. No. 138220)

Antigen Details

Structure
A 205 kD type I tramsmembrane protein belonging to the C-type lectin family and homologous to the macrophage mannose receptor.
Distribution

Expressed at high levels by dendritic cells and thymic epithelial cells. Also expressed by B lymphocytes, macrophages, Langerhans cells, bone marrow cells and epithelial cells.

Function
Mediates endocytosis, antigen uptake, presentation, and cross-presentation to T cells.
Cell Type
B cells, Dendritic cells, Epithelial cells, Langerhans cells, Macrophages
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Jiang WP, et al. 1995. Nature 375:151.
2. Small M and Kraal G. 2003. Int. Immunol. 15:197.
3. Shrimpton RE, et al. 2009. Mol. Immunol. 46:1229.

Gene ID
17076 View all products for this Gene ID
UniProt
View information about CD205 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.20.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account