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APC/Fire™ 750 anti-mouse CD134 (OX-40) Antibody

Pricing & Availability
Clone
OX-86 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRSF4, ACT35, OX-40
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
OX-86_APCFire750_CD134_Antibody_1_081817
Con-A + IL-2 stimulated (3 days) C57BL/6 splenocytes were stained with CD3 Brilliant Violet 421™ and CD134 (clone OX-86) APC/Fire™ 750 (Top) or rat IgG1, κ APC/Fire™ 750 isotype control (Bottom).
  • OX-86_APCFire750_CD134_Antibody_1_081817
    Con-A + IL-2 stimulated (3 days) C57BL/6 splenocytes were stained with CD3 Brilliant Violet 421™ and CD134 (clone OX-86) APC/Fire™ 750 (Top) or rat IgG1, κ APC/Fire™ 750 isotype control (Bottom).
  • OX-86_APCFire750_CD134_Antibody_2_081817
Compare all formats See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Check Availability Save
119422 25 µg 159 CHF
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119423 100 µg 364 CHF
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Description

CD134 is a type I integral membrane protein also known as OX-40, ACT35, and tumor necrosis factor receptor superfamily member 4 (TNFRSF4). This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX-40 receptor binds to the OX-40 ligand (CD252) to provide a costimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, which implies that these interactions are important in the pathogenesis of some autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse OX-40-CD4 chimeric protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone OX-86 has been reported to act as an agonist and stimulate OX-40.

Application References

(PubMed link indicates BioLegend citation)
  1. Higgins LM, et al. 1999. J. Immunol. 162:486. (FC, IHC)
  2. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695. (Costim)
  3. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
RRID
AB_2715992 (BioLegend Cat. No. 119422)
AB_2715993 (BioLegend Cat. No. 119423)

Antigen Details

Structure
TNF receptor superfamily, 50 kD
Distribution

Activated CD4+ and CD8+ T cells, activated B cells.

Function
Receptor for OX-40 ligand, provides co-stimulatory signal for lymphocyte proliferation independent of CD28; thought to play a role in the pathogenesis of some autoimmune diseases
Ligand/Receptor
OX-40 ligand
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695.
2. Weinberg AD, et al. 1999. J. Immunol. 162:1818.
3. Akira H, et al. 1999. J. Immunol. 162:7058.
4. Pippig SD, et al. 1999. J. Immunol. 163:6520.
5. Higgins LM, et al. 1999. J. Immunol. 162:486.

Gene ID
22163 View all products for this Gene ID
UniProt
View information about CD134 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 08.21.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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