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APC/Cyanine7 anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
H57-597_APCCy7_TCRb_Antibody_FC_080113
C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) APC/Cyanine7 (filled histrogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
  • H57-597_APCCy7_TCRb_Antibody_FC_080113
    C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) APC/Cyanine7 (filled histrogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
109219 25 µg 94 CHF
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109220 100 µg 215 CHF
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Dillon T, et al. 2013. Int Immunol. 25:259. PubMed
  2. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  3. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  4. Cibrián D, et al. 2020. J Allergy Clin Immunol. 145:199. PubMed
  5. Xu K, et al. 2021. Immunity. 54(5):976-987.e7. PubMed
  6. Acker A, et al. 2014. PLoS One. 9:110015. PubMed
  7. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  8. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  9. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  10. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  11. Alcaraz-Serna A, et al. 2021. Sci Adv. 7:. PubMed
  12. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  13. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  14. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  15. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  16. Bresque M, et al. 2022. J Biol Chem. :101711. PubMed
  17. Harada Y, et al. 2022. iScience. 25:104021. PubMed
  18. Higa T, et al. 2022. Nat Commun. 13:1500. PubMed
  19. Gordon RA, et al. 2020. PLoS One. 15:e0226396. PubMed
  20. Broeck T, et al. 2013. J Immunol. 190:1982. PubMed
  21. Hirata Y, et al. 2015. PLoS One. 10: 0141785. PubMed
  22. Devos M, et al. 2020. J Exp Med. 217:00:00. PubMed
  23. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  24. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  25. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  26. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  27. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  28. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  29. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  30. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  31. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  32. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  33. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  34. Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed
  35. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  36. de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed
  37. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  38. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  39. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  40. Jin C, et al. 2019. Cell. 176:998. PubMed
  41. Huang L, et al. 2020. Front Immunol. 10:3103. PubMed
  42. Giampazolias E, et al. 2021. Cell. . PubMed
  43. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  44. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  45. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
  46. Yabas M, et al. 2011. PLoS One. 6:e26440. PubMed
  47. Seki N, et al. 2022. iScience. 25:104838. PubMed
  48. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  49. Murata T, et al. 2020. Sci Rep. 10:13560. PubMed
  50. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  51. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  52. Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed
  53. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  54. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  55. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  56. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  57. Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
  58. Wang X, et al. 2022. Elife. 11:. PubMed
  59. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  60. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  61. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  62. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  63. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  64. Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
  65. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_893626 (BioLegend Cat. No. 109219)
AB_893624 (BioLegend Cat. No. 109220)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All TCR β chain Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TCR β chain H57-597 FC
Biotin anti-mouse TCR β chain H57-597 FC,IHC-F
FITC anti-mouse TCR β chain H57-597 FC,IHC-F
PE anti-mouse TCR β chain H57-597 FC
PE/Cyanine5 anti-mouse TCR β chain H57-597 FC
Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
Alexa Fluor® 488 anti-mouse TCR β chain H57-597 FC,IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain H57-597 FC,IHC-F
APC/Cyanine7 anti-mouse TCR β chain H57-597 FC
PE/Cyanine7 anti-mouse TCR β chain H57-597 FC
Alexa Fluor® 700 anti-mouse TCR β chain H57-597 FC
Pacific Blue™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 421™ anti-mouse TCR β chain H57-597 FC,IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 570™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 510™ anti-mouse TCR β chain H57-597 FC,IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready) H57-597 FC,CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain H57-597 FC,IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 605™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 711™ anti-mouse TCR β chain H57-597 FC
APC/Fire™ 750 anti-mouse TCR β chain H57-597 FC
TotalSeq™-A0120 anti-mouse TCR β chain H57-597 PG
Brilliant Violet 785™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 650™ anti-mouse TCR β chain H57-597 FC
Ultra-LEAF™ Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
TotalSeq™-C0120 anti-mouse TCR β chain H57-597 PG
TotalSeq™-B0120 anti-mouse TCR β chain H57-597 PG
Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Go To Top Version: 3    Revision Date: 02.06.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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