APC/Cyanine7 anti-mouse TCR β chain Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Affinity purified TCR from mouse DO-11.10 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain¹². The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation², in vitro stimulation^2,3, in vivo depletion^4-6, and immunohistochemical staining of acetone-fixed frozen sections^7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)

1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.

Product Citations

1. Dillon T, et al. 2013. Int Immunol. 25:259. PubMed
2. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
3. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
4. Cibrián D, et al. 2020. J Allergy Clin Immunol. 145:199. PubMed
5. Xu K, et al. 2021. Immunity. 54(5):976-987.e7. PubMed
6. Acker A, et al. 2014. PLoS One. 9:110015. PubMed
7. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
8. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
9. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
10. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
11. Alcaraz-Serna A, et al. 2021. Sci Adv. 7:. PubMed
12. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
13. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
14. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
15. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
16. Bresque M, et al. 2022. J Biol Chem. :101711. PubMed
17. Harada Y, et al. 2022. iScience. 25:104021. PubMed
18. Higa T, et al. 2022. Nat Commun. 13:1500. PubMed
19. Gordon RA, et al. 2020. PLoS One. 15:e0226396. PubMed
20. Broeck T, et al. 2013. J Immunol. 190:1982. PubMed
21. Hirata Y, et al. 2015. PLoS One. 10: 0141785. PubMed
22. Devos M, et al. 2020. J Exp Med. 217:00:00. PubMed
23. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
24. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
25. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
26. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
27. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
28. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
29. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
30. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
31. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
32. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
33. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
34. Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed
35. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
36. de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed
37. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
38. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
39. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
40. Jin C, et al. 2019. Cell. 176:998. PubMed
41. Huang L, et al. 2020. Front Immunol. 10:3103. PubMed
42. Giampazolias E, et al. 2021. Cell. . PubMed
43. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
44. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
45. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
46. Yabas M, et al. 2011. PLoS One. 6:e26440. PubMed
47. Seki N, et al. 2022. iScience. 25:104838. PubMed
48. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
49. Murata T, et al. 2020. Sci Rep. 10:13560. PubMed
50. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
51. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
52. Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed
53. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
54. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
55. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
56. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
57. Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
58. Wang X, et al. 2022. Elife. 11:. PubMed
59. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
60. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
61. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
62. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
63. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
64. Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
65. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed

RRID

AB_893626 (BioLegend Cat. No. 109219)
AB_893624 (BioLegend Cat. No. 109220)

Antigen Details

Structure

Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit

Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function

Antigen recognition, T cell activation

Ligand/Receptor

Peptide bound-MHC class I and II

Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID

21577 View all products for this Gene ID

UniProt

View information about TCR beta chain on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis