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APC/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
D7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
D7_APCCy7_072210
C57BL/6 splenocytes stained with D7 APC/Cyanine7
  • D7_APCCy7_072210
    C57BL/6 splenocytes stained with D7 APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
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108125 25 µg 101 CHF
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108126 100 µg 311 CHF
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Barros-Silva JD, et al. 2018. Cell Rep. 25:3504. PubMed
  2. Abraham A, et al. 2019. J Clin Invest. 130:2685. PubMed
  3. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  4. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  5. Corna G, et al. 2016. J Immunol. 197: 1914 - 1925. PubMed
  6. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  7. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  8. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  9. Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed
  10. Chen X, et al. 2021. Theranostics. 11:4655. PubMed
  11. Fu X, et al. 2015. J Biol Chem. 290: 26445 - 26456. PubMed
  12. Nawaz A, et al. 2022. Nat Commun. 13:7058. PubMed
  13. Valds-Mora F, et al. 2021. Cell Reports. 35(2):108945. PubMed
  14. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  15. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  16. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  17. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  18. Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed
  19. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  20. Grandl G, et al. 2016. Mol Metab. 5:937-947. PubMed
  21. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  22. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  23. Gomzikova MO, et al. 2020. Sci Rep. 10:10740. PubMed
  24. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  25. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  26. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
  27. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  28. Helbling PM, et al. 2019. Cell Rep. 29:3313. PubMed
  29. Hermetet F, et al. 2019. Nat Commun. 10:523. PubMed
  30. Agarwal P, et al. 2019. Cell Stem Cell. 24:769. PubMed
  31. Ye H, et al. 2021. Cancer Discov. 0.805555556. PubMed
  32. Koyama T, et al. 2022. Curr Issues Mol Biol. 44:3146. PubMed
  33. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  34. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  35. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  36. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  37. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  38. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  39. Ozaki M, et al. 2021. J Stem Cells Regen Med. 16:50. PubMed
  40. Jie Z, et al. 2014. J Immunol. 192:3289. PubMed
  41. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  42. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  43. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
  44. Hu B, et al. 2020. J Clin Invest. 130:3483. PubMed
  45. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  46. Agarwal P, et al. 2021. Cell Reports. 36(2):109386. PubMed
  47. Jatho A, et al. 2022. Cells. 11:. PubMed
  48. Fast EM, et al. 2021. Elife. 10:. PubMed
RRID
AB_10639725 (BioLegend Cat. No. 108125)
AB_10645327 (BioLegend Cat. No. 108126)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 07.11.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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