APC/Cyanine7 anti-mouse F4/80 Recombinant Antibody

Pricing & Availability
QA17A29 (See other available formats)
Regulatory Status
Other Names
EMR1, Ly71
Mouse IgG1, κ
Ave. Rating
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Product Citations
BALB/c mouse peritoneal macrophages were stained with anti-mouse F4/80 recombinant (clone QA17A29) APC/Cyanine7 (filled histogram) or APC/Cyanine7 mouse IgG1, κ isotype control (open histogram).
  • QA17A29_APCCy7_F4slash80_RECOM_Antibody_091621.png
    BALB/c mouse peritoneal macrophages were stained with anti-mouse F4/80 recombinant (clone QA17A29) APC/Cyanine7 (filled histogram) or APC/Cyanine7 mouse IgG1, κ isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
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157315 25 µg 147 CHF
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F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Murine macrophages
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
AB_2894638 (BioLegend Cat. No. 157315)

Antigen Details

A 160 kD glycoprotein and a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family

Expressed on the majority of tissue macrophages

Required for induction of CD8+ T cells-mediated peripheral tolerance
Cell Type
Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035.
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3.
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354.
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077.
  5. Glass AM, et al. 2013. J. Immunol. 190:4830.
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570.
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526.
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. 
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322.
  10. Ohnishi A, et al. 2015. PLoS One. 10:117616.
Gene ID
13733 View all products for this Gene ID
View information about F4/80 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 09.16.2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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