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APC anti-mouse Podoplanin Antibody

Pricing & Availability
Clone
8.1.1 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, gp38, stromal cell marker
Isotype
Syrian Hamster IgG
Ave. Rating
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Product Citations
publications
8.1.1_APC_060710
Mouse thymic epithelial stromal cell line TE-71 stained with 8.1.1 APC
  • 8.1.1_APC_060710
    Mouse thymic epithelial stromal cell line TE-71 stained with 8.1.1 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
127409 25 µg 137 CHF
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127410 100 µg 303 CHF
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Description

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
  2. Farr AG, et al. 1992. J. Exp. Med. 176:1477.
  3. Bekiaris V, et al. 2008. J. Immunol. 180:6768.
  4. Algars A, et al. 2011. Blood 117:4387. PubMed
  5. Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
  6. Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
  7. Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Buechler MB, et al. 2021. Nature. 593:575. PubMed
  3. Weeden C, et al. 2017. PLoS Biol. 10.1371/journal.pbio.2000731. PubMed
  4. Urban SK, et al. 2020. Liver Int. 40:3103. PubMed
  5. McCarthy N, et al. 2020. Cell Stem Cell. 391:26. PubMed
  6. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  7. Seidenberg J, et al. 2021. Int J Mol Sci. :22. PubMed
  8. Brinkman C, et al. 2013. J Immunol. 191:2412. PubMed
  9. Schwab M, et al. 2021. Exp Dermatol. 30:1619. PubMed
  10. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  11. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  12. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  13. Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed
  14. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  15. Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed
  16. Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed
  17. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  18. Denton A, et al. 2014. Proc Natl Acad Sci U S A. 111:12139. PubMed
  19. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  20. Eisemann T, et al. 2019. Front Oncol. 9:187. PubMed
  21. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  22. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  23. Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed
  24. Coulombe P et al. 2019. Cell reports. 27(6):1769-1780 . PubMed
  25. Hara T, et al. 2021. Cancer Cell. 39(6):779-792.e11. PubMed
  26. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  27. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
  28. Tadayon S, et al. 2021. Front Immunol. 12:602122. PubMed
  29. Costa B, et al. 2019. Blood Adv. 3:1092. PubMed
RRID
AB_10612940 (BioLegend Cat. No. 127409)
AB_10613649 (BioLegend Cat. No. 127410)

Antigen Details

Structure
43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution

Expressed on epithelial and mesothelial cells.

Cell Type
Epithelial cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.

Gene ID
14726 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 02.03.2017

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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