APC anti-mouse LAP (TGF-β1) Antibody

Pricing & Availability
Clone
TW7-16B4 (See other available formats)
Other Names
Latency Associated Peptide (LAP), Transforming growth factor-beta 1 (TGF-b1), TGFB1, DPD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TW7-16B4_APC_1_062911
CD3+CD28+IL-2-stimulated C57BL/6 mouse splenocytes (48 hours) were surface stained with CD4 FITC and LAP (clone TW7-16B4) APC (top) or mouse IgG1, κ APC isotype control (bottom), then intracellularly stained with FOXP3 PE. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_APC_1_062911
    CD3+CD28+IL-2-stimulated C57BL/6 mouse splenocytes (48 hours) were surface stained with CD4 FITC and LAP (clone TW7-16B4) APC (top) or mouse IgG1, κ APC isotype control (bottom), then intracellularly stained with FOXP3 PE. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_APC_2_062911
See APC spectral data
Cat # Size Price Quantity Avail. Save
141405 25 µg 115 CHF
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141406 100 µg 295 CHF
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Description

Transforming growth factor beta (TGF-β) is a cytokine that has critical functions in the immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associate peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Tgfb1-transduced P3U1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone TW7-16B4 has been reported to not cross-react with bovine LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-16B4 recognizes recombinant LAP, latent TGF-β, and pro-TGF-β.

Additional reported applications (for relevant formats) include: Western blotting1 and immunoprecipitation1

Application References

(PubMed link indicates BioLegend citation)
  1. Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB)
  2. Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
Product Citations
  1. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  2. Mukhopadhyay D, et al. 2015. PLoS Negl Trop Dis. 9: e0004145. PubMed
  3. Gubser C, et al. 2016. Sci Rep. 6: 25758. PubMed
  4. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  5. Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
  6. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
Publication Library
RRID
AB_10896418 (BioLegend Cat. No. 141405)
AB_10898159 (BioLegend Cat. No. 141406)

Antigen Details

Structure
Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
Distribution
Many cell types, highly expressed on activated Tregs and platelets
Function
TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response
Ligand/Receptor
TGF-β receptors
Cell Type
Tregs, Platelets, Dendritic cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Oida T, et al. 2010. PLoS One 5:e15523.
2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.
5. Nakamura K. 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factors 8:11.

Gene ID
21803 View all products for this Gene ID
UniProt
View information about LAP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08.27.2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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