- P84 (See other available formats)
- Regulatory Status
- Other Names
- SHPS-1, BIT, P84, PTPNS1, CD172 antigen-like family member A
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
|144013||25 µg||125,00 CHF|
|144014||100 µg||285,00 CHF|
CD172a, also known as SIRPα, is a type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. CD172a is expressed by monocytes, macrophages, myeloid cells, and neuronal tissue. The phosphorylation of SIRPα ITIMs induces the recruitment and activation of the tyrosine phosphatases PTPN6 and PTPN11, resulting in the negative regulation of several biological processes. The ligands of CD172a are CD47, SP-A, and SP-D.Product Details
- Antibody Type
- Host Species
- Mouse brain membrane protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD474, in vivo blocking of dendritic cell migration3, enhancing of macrophage phagocytosis2,4, immunohistochemical staining of cerebellum frozen sections1, and immunoprecipitation2,4.
(PubMed link indicates BioLegend citation)
- Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
- Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
- Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
- Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
- Product Citations
AB_2564060 (BioLegend Cat. No. 144013)
AB_2564061 (BioLegend Cat. No. 144014)
- Type I transmembrane protein
Monocytes, macrophages, myeloid cells, neuronal tissue
- Negative regulation of several biological processes
- PTPN6, PTPN11
- CD47, SP-A, SP-D
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
- Antigen References
1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510.
- Gene ID
- 19261 View all products for this Gene ID
- View information about CD172a on UniProt.org
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