Loading...

APC anti-human IL-6 Antibody

Pricing & Availability
Clone
MQ2-13A5 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
MQ2-13A5_APC_031808
LPS-stimulated (6 hours) human peripheral blood monocytes surface stained with CD14 PE/Cy7 and intracellular stained with MQ2-13A5 APC
  • MQ2-13A5_APC_031808
    LPS-stimulated (6 hours) human peripheral blood monocytes surface stained with CD14 PE/Cy7 and intracellular stained with MQ2-13A5 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
501112 100 tests 308 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-7- expressed, recombinant human IL-6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Capture1-3,7: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody.

Neutralization1-3,5,6: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Additional applications (for the relevant formats) include: intracellular flow cytometry10.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
  3. Gaines Das R, et al. 1993. J. Immunol. Methods 160:147.
  4. Enriquez J, et al. 2002. Adv. Perit Dial. 18:177.
  5. Zou JP, et al. 1999. J. Immunol. 162:4882.
  6. Wyant TL, et al. 1999. Infect. Immun. 67:1338.
  7. Lesmeister MJ, et al. 2005. Reprod. Biol. Endocrinol. 3:74.
  8. Terasaka Y, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:2441 PubMed
  9. Girndt M, et al. 1998. J. Am. Soc. Nephrol. 9:1689.
Product Citations
  1. Rizzo MD, et al. 2019. J Pharmacol Exp Ther. 371:191. PubMed
  2. Arra A, et al. 2021. Oncoimmunology. 10:1938475. PubMed
  3. Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
  4. Santos R, et al. 2017. Stem Cell Reports. 1.553472222. PubMed
  5. Biasin M, et al. 2010. J Immunol. 184:2710. PubMed
  6. Hardman CS, et al. 2021. Sci Immunol. 6:. PubMed
  7. Shey M, et al. 2014. J Immunol. 192:4833. PubMed
  8. Piconi S, et al. 2011. Blood. 118:3263. PubMed
  9. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  10. Prakash MD, et al. 2021. PLoS One. 16:e0247492. PubMed
  11. Rizzo MD, et al. 2019. J Neuroimmunol. 333:576969. PubMed
  12. Vivas–García Y, et al. 2020. Molecular Cell. 77(1):120-137.e9.. PubMed
  13. Baguma R, et al. 2017. PLoS One. 10.1371/journal.pone.0184563. PubMed
RRID
AB_389222 (BioLegend Cat. No. 501112)

Antigen Details

Structure
Neuropoietic cytokine family; 26 kD (Mammalian)
Bioactivity
Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets
T cells, B cells, hepatocytes, cholinergic neurons
Receptors
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Int. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. van Oers M, et al. 1993. Ann. Hematol. 66:219.

Regulation
Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, and IL-17; downregulated by glucocorticoids IL-4 and TGF-β
Gene ID
3569 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All IL-6 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-human IL-6 MQ2-13A5 ICFC
PE anti-human IL-6 MQ2-13A5 ICFC
Purified anti-human IL-6 MQ2-13A5 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
APC anti-human IL-6 MQ2-13A5 ICFC
Pacific Blue™ anti-human IL-6 MQ2-13A5 ICFC
Purified anti-human IL-6 (Maxpar® Ready) MQ2-13A5 ELISA Capture,CyTOF®
PerCP/Cyanine5.5 anti-human IL-6 MQ2-13A5 ICFC
PE/Cyanine7 anti-human IL-6 MQ2-13A5 ICFC
Alexa Fluor® 647 anti-human IL-6 MQ2-13A5 ICFC
PE/Dazzle™ 594 anti-human IL-6 MQ2-13A5 ICFC
Ultra-LEAF™ Purified anti-human IL-6 MQ2-13A5 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
Go To Top Version: 2    Revision Date: 07.11.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human IL-6

  • PE anti-human IL-6

  • Purified anti-human IL-6

  • APC anti-human IL-6

  • Pacific Blue™ anti-human IL-6

  • Purified anti-human IL-6 (Maxpar® Ready)

  • PerCP/Cyanine5.5 anti-human IL-6

  • PE/Cyanine7 anti-human IL-6

  • Alexa Fluor® 647 anti-human IL-6

  • PE/Dazzle™ 594 anti-human IL-6

  • Ultra-LEAF™ Purified anti-human IL-6
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account