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APC anti-human CD366 (Tim-3) Antibody

Pricing & Availability
Clone
A18087E (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A18087E_APC_CD366_Antibody_1_122221
PHA-stimulated human peripheral blood lymphocytes were stained with anti-human CD3 PE/Cyanine7 and anti-human CD366 (Tim-3) (clone A18087E) APC (left) or mouse IgG1, ᴋ APC isotype control (right).
  • A18087E_APC_CD366_Antibody_1_122221
    PHA-stimulated human peripheral blood lymphocytes were stained with anti-human CD3 PE/Cyanine7 and anti-human CD366 (Tim-3) (clone A18087E) APC (left) or mouse IgG1, ᴋ APC isotype control (right).
  • A18087E_APC_CD366_Antibody_122221
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with anti-human CD366 (Tim-3) (clone A18087E) APC (filled histogram) or mouse IgG1, ᴋ APC isotype control (open histogram).
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364803 25 tests 154 CHF
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364804 100 tests 355 CHF
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Tim-3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2910409 (BioLegend Cat. No. 364803)
AB_2910409 (BioLegend Cat. No. 364804)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells

Function
CD366 plays a role in regulating macrophage activation, checkpoint inhibitor, T cell apoptosis and immune tolerance
Ligand/Receptor
Galectin-9 and PtdSer
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Hafler DA and Kuchroo V. 2008. J Exp Med. 205:2699.
  2. Zhu C, et al. 2005. Nat Immunol. 6:1245.
  3. Wang F, et al. 2009. Immunobiology. 214:342.
Gene ID
84868 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.22.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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