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Anti-CD243 (ABCB1, P-glycoprotein 1) Antibody (Previously Covance catalog# SIG-38730)

Pricing & Availability
Clone
JSB-1 (See other available formats)
Regulatory Status
RUO
Other Names
P-glycoprotein 1, ABC20, CLCS, GP170, MDR1, P-GP, PGY1, colchicin sensitivity, doxorubicin resistance, multidrug resistance protein 1, ATP-binding cassette, sub-family B (MDR/TAP), member 1, MRP1, Multidrug resistance protein 1, P-glycoprotein 1
Previously
Signet Catalog# 8730-01
Covance Catalog# SIG-38730
Isotype
Mouse IgG1
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Product Citations
publications
JSB-1_Purified_CD243_Antibody_IHC_021315
Staining of CD243 (JSB-1) on formalin-fixed paraffin-embedded normal human liver tissue.
  • JSB-1_Purified_CD243_Antibody_IHC_021315
    Staining of CD243 (JSB-1) on formalin-fixed paraffin-embedded normal human liver tissue.
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901401 1 mL 533 CHF
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Description

CD243 (MDR-1) belongs to the ATP binding cassette (ABC) transporter family. With an approximate molecular mass of 170 kD, it consists of two homologous halves. Each half contains two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs). The TMDs span the membrane six times, forming a chamber with a 12 transmembrane α-helix structure. NBDs drive the transport process through ATP coupling and hydrolysis and are located at the cytoplasmic face of the membrane. CD243 transports various molecules across cellular membranes and is involved in multidrug resistance. MDR-1 is expressed on hematopoietic stem cells, T cells, B cells, and NK cells as well as on many multidrug resistant neoplastic cells. CD243 interacts with Caveolin, RING finger protein 1B, AAP1, p53, Orphan nuclear receptor PX, and cytochrome P450.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a multidrug-resistant Chinese hamster ovary cell line (CHrC5).
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
Tissue Culture Supernatant
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P, ICC, WB, FC
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:40 for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Formalin-fixed, paraffin-embedded tissues.
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (Cat. No. 927901).
Incubation: 60 minutes at room temperature.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC).

ICC Positive Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives.
IHC Positive Tissue: Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretaory epithelium).

JSB-1 identifies an independent, strongly conserved cytoplasmic epitope present on the mammalian MDR1 P-glycoprotein molecule. JSB-1 does not cross-react with MDR3 P-glycoprotein. JSB-1 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant 130kDa mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections. Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein expresssion. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.

Application References

(PubMed link indicates BioLegend citation)
  1. Scheper RJ, et al. 1998. Int. J. Cancer. 42:389.
  2. Schinkel AH, et al. 1991. Cancer Res. 51:2628.
  3. Tiirikainen MI, et al. 1992. Annals Hematol. 65:124.
  4. Goasquen JE, et al. 1993. Blood 81:2394.
  5. Itsubo M, et al. 1994. Cancer 73:298.
  6. Klimecki WT, et al. 1994. Blood 83:2451.
  7. Tiirikainen MI, et al. 1992. Ann. Hematol. 65:124. (FC)
  8. Mahadevan D, et al. 2013. Cancer. 119:371. (IHC) PubMed
  9. Beck WT, et al. 1996. Cancer Res. 56:3010. PubMed
Product Citations
  1. Liu H, et al. 2019. Mol Med Rep. 19:2173. PubMed
  2. Mahadevan D, et al. 2013. Cancer. 119:371-379. PubMed
  3. Beck W, et al. 1996. Cancer Res. 56:3010-3020. PubMed
RRID
AB_2565004 (BioLegend Cat. No. 901401)

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Go To Top Version: 2    Revision Date: 07.19.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Anti-CD243 (ABCB1, P-glycoprotein 1)
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