Alexa Fluor® 700 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
7 reviews
Product Citations
publications
17A2_Alx700_021808
C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 700
  • 17A2_Alx700_021808
    C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Avail. Save
100215 25 µg 85 CHF
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100216 100 µg 195 CHF
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2.

Application References

(PubMed link indicates BioLegend citation)
  1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD)
  2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD)
  4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC-F)
  5. Zan H, et al. 2005. EMBO J. 24:3757.
  6. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
  7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
  8. Konnecke I, et al. 2014. Bone. PubMed
  9. Steelman A, et al. 2013. J Biol Chem. 16:23776 (ICC)
  10. Shi M, et al. 2006. J Immunol. 176:1724 (ICC)
Product Citations
  1. Wojno E, et al. 2011. J Immunol. 187:266. PubMed
  2. Abdul-Careem MF, et al. 2012. PLoS One. 7:e32821. PubMed
  3. Everard A, et al. 2014. Nat Commun. 5:5648. PubMed
  4. Mykkänen O, et al. 2014. PLoS One. 9:114790. PubMed
  5. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  6. Bruder J, et al. 2017. Mol Ther Methods Clin Dev. 10.1016/j.omtm.2017.08.003. PubMed
  7. Zaitseva M, et al. 2017. Antiviral Research. 10.1016/j.antiviral.2017.05.002. PubMed
  8. Watanabe K, et al. 2018. JCI Insight. 3. PubMed
  9. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  10. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  11. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
  12. Hebbandi Nanjundappa R, et al. 2017. Cell. 171:655. PubMed
  13. Rezende RM, et al. 2019. J Immunol. 203:2621. PubMed
  14. Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed
  15. Agashe VV, et al. 2018. J Immunol. 201:772. PubMed
  16. Li Q, et al. 2019. Front Pharmacol. 10:786. PubMed
  17. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  18. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  19. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  20. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  21. Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed
  22. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  23. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  24. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  25. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  26. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  27. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  28. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
Publication Library
RRID
AB_493696 (BioLegend Cat. No. 100215)
AB_493697 (BioLegend Cat. No. 100216)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.27.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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