Alexa Fluor® 647 Streptavidin

Pricing & Availability
Other Names
Streptavidin-Alexa Fluor® 647, Streptavidin-AF647
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Product Citations
publications
Strepavidin_A647_Antibody_FC_013114
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-Alexa Fluor® 647.
  • Strepavidin_A647_Antibody_FC_013114
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-Alexa Fluor® 647.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
405237 100 µg 175 CHF
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Alexa Fluor® 647 is useful for detecting biotinylated antibodies. The excitation of Alexa Fluor® 647 by 633 nm / 635 nm laser light induces a light emission maximum of 668 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin was conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-Alexa Fluor® 647 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Alexa Fluor® 647 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® is a trademark of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Streptavidin-Alexa Fluor® 647 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Alexa Fluor® 647 is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245.
  2. Nishimoto KP,et al. 2008. J. Immunol. 181:4010.PubMed
  3. Niki T, et al. 2009. J. Biol. Chem. 284:32344. PubMed
  4. Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed
  5. Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed
  6. Yamakawa N, et al. 2012. Int Immunol. PubMed
  7. Shibata T, et al. 2012. Int Immunol. 24:613. PubMed
  8. Baccala R, et al. 2012. J. Immunol. 189:5976. PubMed
  9. Grevers LC, et al. 2013. Ann Reheum Dis. 72:278. PubMed
  10. Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed
  11. Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed
  12. Reading JL, 2013. J. immunol. 190:4542. Pubmed.
  13. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed
  14. Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed
  15. Shade KT, et al. 2015. J Exp Med. 212:457. PubMed
Product Citations
  1. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  2. Yoshikawa S, et al. 2016. Sci Rep. 6:18738. PubMed
  3. Pabbisetty S, et al. 2016. Proc Natl Acad Sci U S A. 113: E4662 - E4670. PubMed
  4. Janeczek A, et al. 2017. Nanomedicine. 10.2217/nnm-2016-0386. PubMed
  5. Kulkarni N, et al. 2017. Eur J Appl Physiol.. 10.1007/s00421-017-3704-z. PubMed
  6. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  7. Pobezinskaya EL, et al. 2019. Front Immunol. 10:955. PubMed
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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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