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Alexa Fluor® 647 anti-Tubulin Beta 3 (TUBB3) Antibody

Pricing & Availability
Clone
AA10 (See other available formats)
Regulatory Status
RUO
Other Names
β-3 Tubulin, Neuronal class III beta-tubulin, β3-tub
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_AA10_AF647_TUBB3_Antibody_1_FC_051414
Human lung adenocarcinoma cell line A549 was treated with Fixation Buffer (Cat# 420801) and Permeabilization Wash Buffer (Cat# 421002), and then stained with TUBB3 (clone AA10) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
  • 1_AA10_AF647_TUBB3_Antibody_1_FC_051414
    Human lung adenocarcinoma cell line A549 was treated with Fixation Buffer (Cat# 420801) and Permeabilization Wash Buffer (Cat# 421002), and then stained with TUBB3 (clone AA10) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
  • 2_AA10_AF647_TUBB3_Antibody_2_IHCF_091916
    C57BL/6 mouse frozen brain tissue was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5 % Triton X-100 for ten minutes, and blocked with 5% FBS for 1 hour. Then the tissue was stained with 1.25 µg/mL of Alexa Fluor® 647 anti-Tubulin Beta 3 (TUBB3) (Clone AA10) (shown in red) and 5 µg/mL of Alexa Fluor® 488 anti-GFAP (Clone 2E1.E9) (shown in green) in blocking buffer, overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 10X objective.
  • 3_Still-image_0326-3_20X-1_3
    Formalin-fixed, 300 micron-thick mouse large intestine section was blocked, permeabilized and stained overnight with Tubulin Beta 3 (TUBB3)(clone AA10) Alexa Fluor® 647 (green) at 2.5 µg/mL, Helix NP™ Green (blue), and CD326 (EpCAM)(clone G8.8) Alexa Fluor® 594 (magenta) at 5 µg/mL, optically cleared, and analyzed at 205 μm imaging depth on a confocal microscope. Watch the video.
Compare all formats See Alexa Fluor® 647 spectral data See high resolution IHC-F data...
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657405 25 µg 176 CHF
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657406 100 µg 382 CHF
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Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 1.25-5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Lin YC, et al. 2021. Nat Neurosci. 24:1077. PubMed
  2. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  3. Buljan M, et al. 2020. Molecular Cell. 79(3):504-520. PubMed
RRID
AB_2563609 (BioLegend Cat. No. 657405)
AB_2563610 (BioLegend Cat. No. 657406)

Antigen Details

Structure
450 amino acids with predicted molecular weight of 50 kD
Distribution

Cytosol

Function
Plays important roles in neuronal cell proliferation and differentiation
Interaction
Alpha tubulin, kinesin and dynein
Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References

1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851.
2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015.
3. Locher H, et al. 2013. Differentiation. 85:173.
4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461.
5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726.
6. Koh Y, et al. 2009. Ann. Oncol. 20:1414.

Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 04.13.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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