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Alexa Fluor® 647 anti-mouse IgG2a Antibody

Pricing & Availability
Clone
RMG2a-62 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2a
Isotype
Rat IgG
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Product Citations
publications
a-RMG2a-62_AF647_IgG2a_Antibody_FC_041714.jpg
Human peripheral blood lymphocytes were stained with purified anti-human CD3 (clone HIT3a, mouse IgG2a isotype, filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a (clone RMG2a-62) Alexa Fluor® 647.
  • a-RMG2a-62_AF647_IgG2a_Antibody_FC_041714.jpg
    Human peripheral blood lymphocytes were stained with purified anti-human CD3 (clone HIT3a, mouse IgG2a isotype, filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a (clone RMG2a-62) Alexa Fluor® 647.
  • b-RMG2a-62_A647_IgG2a_Antibody_IHC-P_062123
    IHC staining of Alexa Fluor® 647 anti-mouse IgG2a on formalin-fixed paraffin-embedded human tonsil. Following antigen retrieval using Tris-EDTA, pH 9.0, the tissue was incubated with 5 µg/mL of purified mouse anti-CD45RO (Cat. No. 304202) at 4°C. This was followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG2a (clone RMG2a-62) at 10 µg/mL. Nuclei were counterstained with DAPI (blue, panel A) (Cat. No. 422801) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
407115 25 µg 89 CHF
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407116 100 µg 194 CHF
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Description

The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Gavin AL, et al.2006. Science 314:1936.
  2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
RRID
AB_2563482 (BioLegend Cat. No. 407115)
AB_2563483 (BioLegend Cat. No. 407116)

Related FAQs

IgG2a gene is deleted in some mouse strains, which ones are they?

IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.

Go To Top Version: 3    Revision Date: 06.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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