Alexa Fluor® 647 anti-human TRA-1-60-R Antibody

Pricing & Availability
TRA-1-60-R (See other available formats)
Regulatory Status
Other Names
Podocalyxin, TRA-1
Mouse IgM, κ
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Product Citations
NCCIT (human teratocarcinoma cell line) stained with TRA-1-60-R Alexa Fluor® 647 (red line) or mouse IgM, κ Alexa Fluor® 647 isotype control (blue line).
  • Tra-1-60-R_A647_102108.jpg
    NCCIT (human teratocarcinoma cell line) stained with TRA-1-60-R Alexa Fluor® 647 (red line) or mouse IgM, κ Alexa Fluor® 647 isotype control (blue line).
Compare all formats See Alexa Fluor® 647 spectral data
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330605 25 tests 165 CHF
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330606 100 tests 347 CHF
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TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R).  The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity.  Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human embryonal carinoma cell line 2102Ep cl.2A6
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, immunohistochemical staining, acetone-fixed frozen sections, immunoprecipitation, and radioimmunoassay.

Application References

(PubMed link indicates BioLegend citation)
  1. Miyoshi N, et. al. 2010. P. Natl. Acad. Sci. USA 107:40. (IF)
  2. Kuai XL, et al. 2009. Differentiation 77:229. (WB)
  3. King F, et al. 2009. Stem Cells Dev. 18:1441. (FC)
Product Citations
  1. Keller AL, et al. 2021. Stem Cell Research. 54:102427. PubMed
  2. Telias M, et al. 2013. Dev Biol. 374:32. PubMed
  3. Chung K, et al. 2014. PLoS One. 9:95304. PubMed
  4. Castro-Gutierrez R, et al. 2021. Front Endocrinol (Lausanne). 12:707881. PubMed
  5. Mair B et al. 2019. Cell reports. 27(2):599-615 . PubMed
  6. Klaihmon P, et al. 2021. Stem Cell Research. 55:102472. PubMed
AB_1227813 (BioLegend Cat. No. 330605)
AB_1227812 (BioLegend Cat. No. 330606)

Antigen Details


Embryonic stemcells

Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Gene ID
5420 View all products for this Gene ID
View information about TRA-1-60-R on

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Go To Top Version: 2    Revision Date: 06.27.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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