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Alexa Fluor® 647 anti-human CD71 Antibody

Pricing & Availability
Clone
CY1G4 (See other available formats)
Regulatory Status
RUO
Workshop
A015
Other Names
T9, Transferrin receptor
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
CY1G4_A647_CD71_Antibody_041317
PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD71 (clone CY1G4) Alexa fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa fluo® 647 isotype control (open histogram).
  • CY1G4_A647_CD71_Antibody_041317
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD71 (clone CY1G4) Alexa fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa fluo® 647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
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334117 25 tests 112 CHF
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334118 100 tests 223 CHF
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Description

CD71 is a 95 kD type II homodimeric transmembrane glycoprotein also known as T9 and transferrin receptor. It is expressed on proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NALM-6 pre-B cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Schank M, et al. 2020. Cell Death Dis. 1.173611111. PubMed
  2. Zhao J, et al. 2021. Front Immunol. 12:658420. PubMed
  3. Schank M, et al. 2021. Front Immunol. 12:760707. PubMed
  4. Li TJ, et al. 2021. Int J Cancer. 148:1756. PubMed
  5. Chou DB, et al. 2022. Nat Commun. 13:6021. PubMed
RRID
AB_2687104 (BioLegend Cat. No. 334117)
AB_2687105 (BioLegend Cat. No. 334118)

Antigen Details

Structure
Transferrin receptor family, type II glycoprotein, 95 kD
Distribution

Proliferating cells, reticulocytes, erythroid precursors

Function
Mediates uptake of iron
Ligand/Receptor
Transferrin
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Hentze M, et al. 1996. P. Natl. Acad. Sci. USA 93:8175.
2. Trowbridge I, et al. 1993. Annu. Rev. Cell Biol. 9:129.

Gene ID
7037 View all products for this Gene ID
UniProt
View information about CD71 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 04.13.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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