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Alexa Fluor® 647 anti-human CD22 Antibody

Pricing & Availability
Clone
HIB22 (See other available formats)
Regulatory Status
RUO
Workshop
V CD22.14
Other Names
BL-CAM, Siglec-2, Lyb8
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB22_A647_CD22_Antibody_091117
Human peripheral blood lymphocytes were stained with CD22 (clone HIB22) Alexa Fluor® 647 (filled histogram) or Mouse IgG21, κ Alexa Fluor® 647 isotype control (open histogram).
  • HIB22_A647_CD22_Antibody_091117
    Human peripheral blood lymphocytes were stained with CD22 (clone HIB22) Alexa Fluor® 647 (filled histogram) or Mouse IgG21, κ Alexa Fluor® 647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
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302517 25 tests 112 CHF
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302518 100 tests 276 CHF
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Description

CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. It is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York.
  2. Clark E. 1993. J. Immunol.. 150:4715.
  3. Shan D and O. Press. 1995. J. Immunol.. 154:4466.
Product Citations
  1. Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
  2. Kaewchim K, et al. 2021. Molecules. 26:. PubMed
RRID
AB_2716167 (BioLegend Cat. No. 302517)
AB_2716168 (BioLegend Cat. No. 302518)

Antigen Details

Structure
Ig superfamily, a type I glycosylated integral transmembrane protein, 120-130 kD
Distribution

B cells

Function
Adhesion, B-monocyte and B-T interactions
Ligand/Receptor
CD45RO, CD75
Cell Type
B cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References

1. Clark E. 1993. J. Immunol. 150:4715.
2. Shan D, et al. 1995. J. Immunol. 154:4466.

Gene ID
933 View all products for this Gene ID
UniProt
View information about CD22 on UniProt.org
Go To Top Version: 0    Revision Date: 09.11.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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