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Alexa Fluor® 488 anti-Myelin Basic Protein Antibody

Pricing & Availability
Clone
P82H9 (See other available formats)
Regulatory Status
RUO
Other Names
MBP, Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_P82H9_A488_Myelin_Basic_Protein_Antibody_1_020419.png
IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein antibody (clone P82H9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No.928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 1_P82H9_A488_Myelin_Basic_Protein_Antibody_1_020419.png
    IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein antibody (clone P82H9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No.928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 2_P82H9_A488_Myelin_Basic_Protein_Antibody_2_020419.png
    IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein antibody (clone P82H9) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No.928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 3_P82H9_A488_Myelin_Basic_Protein_Antibody_3_020419.png
    IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein antibody (clone P82H9) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No.928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 4_1_20X_hippo_Maximumintensityprojection
    Formalin-fixed, 300 micron-thick mouse brain (dentate gyrus) section was blocked, permeabilized and stained overnight with Myelin Basic Protein (clone P82H9) Alexa Fluor® 488 (green) and MAP2 (clone SMI 52) Alexa Fluor® 647 (magenta) both at 5 µg/mL, optically cleared, and analyzed on a confocal microscope.
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
850907 25 µg 159 CHF
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850908 100 µg 323 CHF
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Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant MBP protein fragment corresponding to amino acid residues 1-197
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2801191 (BioLegend Cat. No. 850907)
AB_2801192 (BioLegend Cat. No. 850908)

Antigen Details

Structure
Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD.
Distribution

Tissue distribution: Central and peripheral nervous systems
Cellular distribution: Cytoskeleton, plasma membrane, cytosol, and extracellular

Function
Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID
4155 View all products for this Gene ID
UniProt
View information about Myelin Basic Protein on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04.13.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Myelin Basic Protein

  • HRP anti-Myelin Basic Protein

  • Alexa Fluor® 594 anti-Myelin Basic Protein

  • Alexa Fluor® 488 anti-Myelin Basic Protein

  • Alexa Fluor® 647 anti-Myelin Basic Protein
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