Alexa Fluor® 488 anti-mouse Podoplanin Antibody

Pricing & Availability
Clone
PMab-1 (See other available formats)
Other Names
T1a, gp38, stromal cell marker
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
1_PMab-1_A488_Podoplanin_Antibody_081519
Mouse TE-71 cell line was stained with anti-mouse podoplanin (clone PMab-1) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ isotype control (clone RTK2758) Alexa Fluor® 488 (open histogram).
  • 1_PMab-1_A488_Podoplanin_Antibody_081519
    Mouse TE-71 cell line was stained with anti-mouse podoplanin (clone PMab-1) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ isotype control (clone RTK2758) Alexa Fluor® 488 (open histogram).
  • 2_PMab-1_A488_Podoplanin_IHC-F_Antibody_091319
    C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10µg/ml of Alexa Fluor® 488 anti-mouse podoplanin (clone PMab-1, green), 10 µg/ml of Alexa Fluor® 594 anti-mouse CD326 (clone G8.8, red) at 4°C overnight. The image was scanned with a 10X object and stitched with MetaMorph® software.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
156207 25 µg 150 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
156208 100 µg 315 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Peptide (38-51 amino acids of mouse podoplanin)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IHC-F - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Yamada S, et al. 2017. Monoclon Antib Immunodiagn Immunother. 36(2):77-79.
RRID
AB_2814079 (BioLegend Cat. No. 156207)
AB_2814080 (BioLegend Cat. No. 156208)

Antigen Details

Structure
43 kD glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution

Expressed on epithelial and mesothelial cells.

Function
Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation.
Interaction
Interacts with CLEC1B; the interaction is independent of CLEC1B glycosylation and activates CLEC1B; the interaction is dependent of sialic acid on O-glycans.
Ligand/Receptor
CLEC1B
Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Farr A, et al. 1992. J Histochem Cytochem. 40:651.
  2. Schacht V, et al. 2005. Am J Pathol. 166:913.
Gene ID
14726 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.15.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account