Alexa Fluor® 488 anti-human SSEA-4 Antibody

Pricing & Availability
MC-813-70 (See other available formats)
Regulatory Status
Other Names
Stage-Specific Embryonic Antigen-4, SSEA4
Mouse IgG3, κ
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Product Citations
NCCIT (human teratocarcinoma cell line) stained with MC-813-70 Alexa Fluor® 488
  • MC-813-70_Alx488_090208.jpg
    NCCIT (human teratocarcinoma cell line) stained with MC-813-70 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
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330411 25 tests 165 CHF
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330412 100 tests 370 CHF
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The MC813-70 antibody reacts with a carbohydrate antigen which is named as Stage-Specific Embryonic Antigen-4 (SSEA-4). It is a terminal structure of globo-series glycolipids (NeuAcα2-3Galβ1-3GalNAcβ1-R). SSEA-4 is expressed on human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos, subset of mesenchymal stem cells, erythrocytes, and erythromyeloid leukemia cell line K562. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-4 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Reported Reactivity
Mouse, Baboon, Cynomolgus, Rhesus
Antibody Type
Host Species
Human embryonic carcinoma cell line 2102Ep
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA); may contain stabilizer.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA1 and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Kannagi R, et al. 1983. The EMBO J. 2:2355
Product Citations
  1. Keller AL, et al. 2021. Stem Cell Research. 54:102427. PubMed
  2. Pacini S, et al. 2010. PLoS One. 5:e9861. PubMed
  3. Fazzi R, et al. 2011. PLoS One. 6:e25600. PubMed
  4. V ölkner C, et al. 2020. Stem Cell Research. 41:101606.. PubMed
  5. Chen D, et al. 2020. Stem Cell Research. 72.73680556. PubMed
  6. Tian P, et al. 2022. Front Cell Dev Biol. 10:835321. PubMed
AB_1089199 (BioLegend Cat. No. 330411)
AB_1089198 (BioLegend Cat. No. 330412)

Antigen Details

Carbohydrate epitope on glycosphingolipids

Human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), preimplantation embryos, and erythrocytes; mouse oocytes, early cleaved embryos

Regulation of differentiation
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References

1. Kannagi R, et al. 1983. The EMBO J. 2:2355
2. Henderson JK, et al. 2002. Stem Cells. 20:329
3. Brimble SN, et al. 2007. Stem Cells. 25:54
4. Battula VL, et al. 2007. Differentiation. 75:279

Gene ID
View information about SSEA-4 on

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Go To Top Version: 3    Revision Date: 03.26.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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