Alexa Fluor® 488 anti-HDAC1 Phospho (Ser406) Antibody

Pricing & Availability
Clone
BT-15 (See other available formats)
Other Names
Histone Deactylase 1, RPD3L1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
BT-15_A488_HDAC1-phospho_Antibody_1_031820.png
Untreated HeLa cells (left) and HeLa cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with anti-HDAC1 Phospho (Ser406) antibody (clone BT-15) Alexa Fluor® 488 and DAPI.
  • BT-15_A488_HDAC1-phospho_Antibody_1_031820.png
    Untreated HeLa cells (left) and HeLa cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with anti-HDAC1 Phospho (Ser406) antibody (clone BT-15) Alexa Fluor® 488 and DAPI.
  • BT-15_A488_HDAC1-phospho_Antibody_2_031820.png
    Untreated HELA cells (left) and HELA cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with anti-HDAC1 Phospho (Ser406) antibody (clone BT-15) Alexa Fluor® 488 and anti-Histone H3-Phosphorylated (Ser28) antibody (clone HTA28) Alexa Fluor® 647.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
611005 25 tests 145 CHF
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611006 100 tests 315 CHF
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Description

Histone Deacetylase 1 (HDAC1) plays a critical role in various cellular processes, including cell cycle progression, proliferation, and differentiation. The enzyme functions by removing acetyl moieties from histone targets, resulting in histone compaction and alterations in nucleosomal positioning.  Aurora kinases phosphorylate HDAC1 at Ser406 during prophase, immediately after cells begin mitosis, resulting in reduced deacetylase activity of HDAC1. This modification plays an essential role in regulating cell cycle progression, as well as controlling the expression of genes involved in central nervous system development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Zebrafish2
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site.

This clone recognizes zebrafish HDAC1 phosphorylated at Ser4062.

Application References

(PubMed link indicates BioLegend citation)
  1. Segre CV, et al, 2016. mAbs. 8: 37-42
  2. Loponte S, et al, 2016. Sci Rep, 6: 30213.
RRID
AB_2861046 (BioLegend Cat. No. 611005)
AB_2861047 (BioLegend Cat. No. 611006)

Antigen Details

Structure
HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD.
Distribution

Nucleus/Ubiquitously expressed

Function
Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Segre CV, et al, 2016. mAbs. 8: 37-42.
  2. Loponte S, et al, 2016. Sci Rep, 6: 30213.
Gene ID
3065 View all products for this Gene ID
UniProt
View information about HDAC1 Phospho Ser406 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.17.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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