Product: Monensin Solution (1,000X) |
Catalog No.: 420701 |
Mariana Mata, Research Assistant Rush University Medical Center |
Monensin Review for IC stain | |||
Overall: | Product Quality: | Ease of Use: | |
Good golgi stop, keeps stain clear. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | T cells, rats splenocytes |
Brief Protocol: | 1. In a 24 well plate, add 1mL of splenocytes at 4x106cells/mL (2x) and 1mL Monensin buffer at 2uL/mL(2x) (final dilution in plate, after adding cells, will be 1X). 2. Incubate cells and golgi stop for 5hrs at 37ËšC. End of stimulation. 3. Harvest cells and wash with FACS buffer twice. |
Results Summary: | Good results for IC stains. |
Additional Notes: |
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