Application: |
Cell Culture |
Cells used: |
Lineage negative Bone Marrow (CD3e-, Ly-6C/G-, CD11b-, CD45R/B220, TER-119-) |
Brief Protocol: |
1. Isolate bone marrow 2. Remove RBC with lysis buffer 3. Deplete Lineage+ cells with magnetic depletion 4. Count and suspend in mem-α containing 20% FBS, mSCF 50ng/mL, mIL3 10ng/mL , mIL6 20ng/mL (R&D), mIL7 25ng/mL (R&D) 5. Add 5000 cells/well (24 well plate) 6. Infect with lentivirus MOI=100 and polybrene=6 ug/mL |
Results Summary: |
Cell viability was equal to current recombinant murine IL-3 from Peprotech. |
Additional Notes: |
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