Product: Zombie UV™ Fixable Viability Kit |
Catalog No.: 423107 |
Sibiao Yue, Predoc Fellow Carnegie Institution for Science |
Great Zombie UV Fixable Viability Kit | |||
Overall: | Product Quality: | Ease of Use: | |
Works perfectly for distinguishing live/dead cells under fixation conditions. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | 8-week old mouse Splenocytes (thawed from nitrogen tank) |
Brief Protocol: | 1) Aliquot thawed cells in 10^6/100ul PBS 2) Add 1 ul Zombie UV and mix well, room temp for 15 mins 3) Wash with 1 ml PBS one time 4) Surface stain and fix for intracellular staining process |
Results Summary: | Clearly separated live and dead cells. |
Additional Notes: | Follow the exact protocol. Keep in mind, no proteins during Zombie staining process. |
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Technical Service Notes |
This customer is correct. Zombie should not be incubated with any additional proteins or antibodies as it will bind to primary amines. |
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