Product: Alexa Fluor® 488 anti-mouse Ly-6G/Ly-6C (Gr-1) |
Catalog No.: 108417 |
Patrick Murphy, Graduate Student University of Rochester |
Gr1-Alexa Fluor® 488 | |||
Overall: | Product Quality: | Ease of Use: | |
Gr1-Alexa Fluor® 488 acceptably identifies Gr1hi and Gr1int populations. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Inflammed Peritoneal Lavage Cells (Murine) |
Brief Protocol: | Fc block for 10 minutes with 1:100 anti-CD16/32. Stain with 1:200 Gr1-Alexa Fluor® 488 and 1:400 CD11b-APC for 30 mins on ice. Wash once. Resuspend in 300ul FACS Buffer and analyze on FACSCalibur. |
Results Summary: | Gr1int (monocytes) and Gr1hi (Neutrophils) could be adequately identified. |
Additional Notes: |
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