Product: PE/Cyanine7 anti-mouse CD8a

Catalog No.: 100722

Robert Yuan, Graduate Student Stanford

Antibody Stains Intestinal CD8a+ T-cells Very Well
Overall:	Product Quality:	Ease of Use:
The anti-CD8a antibody distinguishes the CD8a+ population of T-cells very clearly.

Experimental Design

Application:	Flow Cytometry
Cells used:	Intraepithelial lymphocytes
Brief Protocol:	Intraepithelial lymphocytes were collected from the small intestine by washing the intestinal tissue in a buffer containing EDTA. The cells were then blocked with Fc Block, stained with antibodies, and read on a flow cytometer.
Results Summary:	The CD8a antibody clearly stains the CD8a+ population of T-cells in the intraepithelial lymphocyte fraction very well.
Additional Notes:	The cells in the image shown have been pre-gated for CD3+ cells.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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