Product: FITC anti-human CD25 |
Catalog No.: 302603 |
Jim Hsiao, Staff Scientist Washington University in St. Louis |
FITC anti-human CD25 | |||
Overall: | Product Quality: | Ease of Use: | |
Low amount of CD4+CD25+ cells were found in human PBMC. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Primary human PBMC |
Brief Protocol: | Single cell suspensions of human PBMCs were blocked with Fc block for 30 minutes. Cells were then stained with the FITC-conjugated anti-human CD25 antibody (2.5ug) in FACS buffer for 30 minutes on ice. Cells were then briefly washed and analyzed by flow cytometry. |
Results Summary: | A small population of human PBMC CD4+ cells expressed CD25. |
Additional Notes: |
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