||In vitro cultured CD8 T cells with DCs
||1. Primary splenic CD8 T cells were isolated from mouse spleen. 2. CD8 T cells were diluted in RPMI medium (without FCS!!) to a concentration of 1 Ã¢Ë†â„¢ 107 cells per ml. 3. 1 Ã‚ÂµL of 5 mM Tag-it Violet™ stock solution per ml cells suspension was used. 4. Cells were incubated at 37 °C for 20 min. 5. Staining was stopped by adding 10 times the original staining volume of RPMI medium containing 10 % FCS.6. After centrifugation cells were used for proliferation assay in vitro.
||Excellent cell tracer dye to follow cell divisions in cell culture in vitro.