Product: True-Nuclear™ Transcription Factor Buffer Set

Catalog No.: 424401

Nguyet Minh Hoang, Research Assitant MD Anderson Cancer Center

True-Nuclear™ Transcription Factor Buffer Set Works Well
Overall:	Product Quality:	Ease of Use:
For staining with CD25 PE and Foxp3 Alexa Fluor® 647.

Experimental Design

Application:	Distinguish Tregs by flow cytometry
Cells used:	Primary T-cells
Brief Protocol:	T-cells was isolated from healthy donor and cultured in low dose IL-2 for one week. Cells were then stained with CD25 PE (Clone W6P3) in staining buffer and proceed to stain with True Nuclear Buffer Set standard protocol using Foxp3 Alexa Fluor® 647 (Clone 206D).
Results Summary:	Successfully distinguish T-reg, no compensation needed, very little background.
Additional Notes:	I reduced the volume of Fix and Perm solution by half. Other reviews said it does not work as well at the Foxp3 Buffer, I have not worked with the Foxp3 buffer to compare which one works better.

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