Application: |
Distinguish Tregs by flow cytometry |
Cells used: |
Primary T-cells |
Brief Protocol: |
T-cells was isolated from healthy donor and cultured in low dose IL-2 for one week. Cells were then stained with CD25 PE (Clone W6P3) in staining buffer and proceed to stain with True Nuclear Buffer Set standard protocol using Foxp3 Alexa Fluor® 647 (Clone 206D). |
Results Summary: |
Successfully distinguish T-reg, no compensation needed, very little background. |
Additional Notes: |
I reduced the volume of Fix and Perm solution by half. Other reviews said it does not work as well at the Foxp3 Buffer, I have not worked with the Foxp3 buffer to compare which one works better. |
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