Product: PE anti-mouse IL-22

Catalog No.: 516404

Karine Serre, PhD Instituto de Medicina Molecular - iMM

anti-mouse IL-22-PE Brightly Stains CD27- Gamma Delta T Cells Restimulated for OVN with IL-1b+IL23 Followed by 4h with PMA - Iono
Overall:	Product Quality:	Ease of Use:
Anti-mouse IL-22-PE brightly stains CD27- gamma delta T cells restimulated for OVN with IL-1b+IL23 followed by 4h with PMA - Iono.

Experimental Design

Application:	Flow Cytometry
Cells used:	Cell suspensions from mouse lymphoid tissues such as spleen and lymph nodes.
Brief Protocol:	We isolated cells from peripheral lymphoid tissues, pooled spleen and lymph nodes from C57BL/6 mice, and FACS-sorted CD27- gd T cells. The CD27- gd T cells were stimulated overnight with IL-1b and IL-23, and then 4h with PMA and ionomycin for 4h at 37C. Brefeldin A was added for the last 2h of re-stimulation. Then we performed an intracellular FACS staining using anti-mouse IL-22-PE antibody. We usually put up to 5 x 10^6 cells in a 50ul volume and add anti-mouse IL-22-PE diluted at 1/100 final in permeabilization buffer for 1h at room temperature.
Results Summary:	Anti-mouse IL-22-PE antibody provides a strong signal that allows reliable staining to study gd T cells.
Additional Notes:	Figure shows results from CD27- gamma delta T cells purified from spleen and lymph node of wild type C57BL6 mice and stimulated overnight with IL-1b+IL-23 and then re-stimulated for 4h with PMA/Iono. Cells were stained with anti-IL-22-PE and anti-IL17A-FITC.

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Publication Status This data has been published. Pubmed

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