Product: Zombie Violet™ Fixable Viability Kit

Catalog No.: 423114

Karine Serre, PhD Instituto de Medicina Molecular - iMM

Zombie Violet (Fixable Viability Kit) Excludes Dead Cells After Collagenase DNase Treatment
Overall:	Product Quality:	Ease of Use:
Zombie Violet is a reproducible dye to exclude dead cells in particular after collagenase DNase treatment

Experimental Design

Application:	Flow Cytometry
Cells used:	Cell suspensions from various mouse non-lymphoid tissues including: brain, lung, liver, and tumour
Brief Protocol:	We isolated cells from tumour implanted in from C57BL/6 mice, by using collagenase DNase treatment, and stained them with Zombie Violet dye to exclude dead cells. We put up to 5 x 10^6 cells in a 50ul volume and add Zombie Violet diluted at a 1/1000 final in PBS for 15min at 4C.
Results Summary:	Zombie Violet provides a bright signal that allows strong and reliable staining to exclude dead / dying cells, from various types of organs from mouse.
Additional Notes:	Our laboratory has interest in tumour-infiltrating immune cells and we have tested many different products to exclude dead cells after collagenase DNase treatment. We choose to use routinely Zombie Violet dye as this was the best for our combination with more 9 other colours. Figure shows zombie violet against CD45 staining from E0771 tumour cell suspension of C57BL6 mouse.

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