Product: PE anti-mouse CD19 |
Catalog No.: 115508 |
Christian Maenne, PhD student German Rheumatism Research Centre |
Good Murine CD19 FACS Antibody | |||
Overall: | Product Quality: | Ease of Use: | |
Nice staining Ab for CD19+ B-cells in tissue. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Live Spleenocytes, blood and bone marrow cells |
Brief Protocol: | Single cell suspension from tissue, RBC lysis, stain 2E+06 cells in 50 µl with Ab mix (1:200) on ice wash centrifuge and FACS. |
Results Summary: | Nice separation of CD19+ B-cells in different tissues. |
Additional Notes: | Cells were gated on viable PI- cells in Spleen, blood and BM. |
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