Veri-Cells™...

Are most appropriate when you need reference/control cells for longitudinal, multi-center studies.

Products

Reference laboratories, clinical research organizations, and multi-center clinical trials, among other institutions, need reliable controls to monitor assay performance and variability for longitudinal studies. Veri-Cells™ is our line of lyophilized control cell products, which can be used to help monitor data quality and aid in reproducibility in multi-center and longitudinal studies. 

Veri-Cells™ feature:

  • Exceptional long term stability
  • Scatter profile similar to fresh samples
  • Validation for the detection of over 150 markers

For more helpful information on Veri-Cells™, visit the FAQ page.

 

Veri-Cells™ Heavy Metal (Ta) PBMC

Veri-Cells™ Heavy Metal (Ta) PBMCs have been labeled with the heavy metal tantalum. When mixed with sample cells, they can be separated from the cells of interest using the tantalum signal. They have been verified to stain for commonly tested surface markers including CD3, CD4, CD8, CD14, CD16, CD19, CD38, CD123, and HLA-DR.

Veri-Cells™ Heavy Metal (Ta) PBMCs were mixed with whole blood from different donors. Mixed samples were stained with a heavy-metal labeled anti-CD14 antibody and run on a CyTOF®. Median intensity of CD14 signal was measured for the blood sample alone (top row) and normalized to the median intensity of CD14 signal on Veri-Cells™ in each sample (bottom row). Normalized median values are indicated. Data provided by Adeeb Rahman, PhD, Icahn School of Medicine at Mt. Sinai.


Veri-Cells™ Activated (Cytokine) PBMC

Veri-Cells™ Activated (Cytokine) PBMC have been activated with our Cell Activation Cocktail (with Brefeldin A), which includes phorbol 12-myristate 13-acetate (PMA) and Ionomycin, for 6 hours. They have been validated to stain for activation-induced cytokines including IL-2, IL-3, IL-4, IL-5, IL-8, IL-13, IL-17, GM-CSF, IFN-γ, and TNF-α, along with surface markers typically expressed on human PBMCs. Cells do not require additional fixation nor permeabilization prior to cytokine staining, thus saving more time in your experiment.

 

Veri-Cells™ Activated (Cytokine) PBMCs were stained with anti-CD3 FITC and either anti-TNF-α PerCP/Cyanine5.5, anti-IL-17A BV421™, anti-GM-CSF Pacific Blue™, or anti-IFN-γ APC. Plots were gated based on staining with the corresponding isotype controls as indicated.


Veri-Cells™ Activated (Surface)

Veri-Cells™ Activated (Surface) have been activated with Concanavalin A for 72 hours. This makes them ideal for the detection of T cell activation markers, including CD25, CD69, CD134, CD279, CD366, and TIGIT.

Veri-Cells™ Activated (Surface) PBMCs were stained with anti-CD4 FITC and either anti-CD25 PerCP/Cy5.5, anti-CD134 APC, anti-CD69 APC, or anti-TIGIT APC. Plots were generated on CD3+ cells and gated based on staining with the corresponding isotype control as indicated.


Veri-Cells™ Phospho PBMC (MAPK/ERK Pathway)

Veri-Cells™ Phospho PBMC are stimulated with PMA/Ionomycin for 15 minutes. This product includes both stimulated and vehicle control cells for the staining of RAS mitogen-activated kinase pathways, including phospho-S6, phospho-p38, and phospho-ERK1/2.

 

Veri-Cells™ Phospho PBMC (MAPK/ERK Pathway) vehicle control (open histogram) or stimulated cells (closed histogram) were stained with anti-phospho S6-Alexa Fluor® 488 (left), anti-phospho p38-PE (center) or anti-human phospho ERK-Alexa Fluor® 647 (right). The histograms are gated on the lymphocyte population.


Veri-Cells™ CD34 PBMC

Veri-Cells™ CD34 PBMC contains mixture of CD34+ KG-1a cells and human PBMCs to help verify CD34 antibody binding without the need for artificial enrichment or the collection of a large number of events.

 

Veri-Cells™ CD34 PBMCs were stained with anti-CD45 (clone HI30) FITC and anti-CD34 (clone 561) PE. Dot plots have excluded doublets from analysis.


Veri-Cells™ Leukocytes 

Unlike Veri-Cells™ PBMC and CD4-Low PBMC, Veri-Cells™ Leukocytes contain neutrophils and eosinophil populations. These cells can be used as controls or reference material to monitor expression of granulocyte markers such as CD15 and CD16.

 

Veri-Cells™ Leukocytes were surface stained with anti-CD8 Alexa Fluor® 700, then fixed and permeabilized before staining with anti-Granzyme B FITC, and anti-Perforin PE. Scatter profile of the cells is shown on the left.


Veri-Cells™ CD4-Low PBMC

Veri-Cells™ CD4-Low PBMC are specially formulated to contain a lower frequency of CD4+ cells, similar to that observed in patients with CD4 immunodeficiency. The expected frequency of CD3+ CD4+ cells can be found on the certificate of analysis for that lot.

 

Veri-Cells™ PBMCs and CD4-low PBMCs were stained with anti-CD4 PE/Cy7 and anti-CD3 FITC and the frequency of CD4+ cells is indicated. Plots were first gated on the lymphocyte population.


Veri-Cells™ PBMC

Veri-Cells™ PBMC consist of lyophilized human PBMCs validated for over 150 surface markers and intracellular targets and exhibit a scatter profile similar to freshly isolated cells. Please refer to the Validation Tab to view the full listing of markers and clones that can be used with Veri-Cells™ PBMC.

Stability

Veri-Cells™ are highly stable, whether used immediately, 5 days after reconstitution, or up to a year in its lyophilized state, making it perfect for long-term longitudinal studies.

Consistent cell frequences pre and post-lyophilization

 

Fresh PBMCs and reconstituted Veri-Cells™ PBMC were surface stained with anti-human CD3, CD19 and CD56/CD16 antibodies. The percentage of positive cells was similar before and after the lyophilization process.

Stability after reconstitution

 

Veri-Cells™ PBMC were surface stained with anti-human CD4 PE/Cy7, CD8 APC, CD19 PerCP/Cy5.5, CD56 FITC and CCR6 BV421™ on day 0 and day 5 post-reconstitution.

Long-term stability

 

Frequencies of CD3, CD4, CD8 positive T cells, and CD19 positive B cells were almost identical between Veri-Cells™ PBMC tested immediately post lyophilization and lyophilized Veri-Cells™ PBMC stored at room temperature for 18 months.

Excellent Surface Staining

Excellent Surface Staining

Transcription Factor Staining

Transcription Factor Staining

150+ Surface Markers Validated on Veri-Cells™ PBMC

The following cell surface markers were validated using BioLegend's LEGENDScreen™ Human PE kit.

 

 

Marker Clone
CD1c L161
CD1d 51.1
CD2 RPA-2.10
CD3 HIT3a
CD4 RPA-T4
CD5 UCHT2
CD6 BL-CD6
CD7 CD7-6B7
CD8a HIT8a
CD9 HI9a
CD11a HI111
CD11b ICRF44
CD11b CBRM1/5
CD11c 3.9
CD13 WM15
CD14 M5E2
CD16 3G8
CD18 TS1/18
CD19 HIB19
CD20 2H7
CD21 Bu32
CD22 HIB22
CD23 EBVCS-5
CD24 ML5
CD26 BA5b
CD27 O323
CD28 CD28.2
CD29 TS2/16
CD31 WM59
CD32 FUN-2
CD33 WM53
CD35 E11
CD36 5-271
CD38 HIT2
CD39 A1
CD40 HB14
CD41 HIP8
CD42b HIP1
CD43 CD43-10G7
CD44 BJ18
Marker Clone
CD45 HI30
CD45RA HI100
CD45RB MEM-55
CD45RO UCHL1
CD46 TRA-2-10
CD47 CC2C6
CD48 BJ40
CD49d 9F10
CD49e NKI-SAM-1
CD49f GoH3
CD50 CBR-IC3/1
CD52 HI186
CD53 HI29
CD54 HA58
CD55 JS11
CD56 HCD56
CD57 HCD57
CD58 TS2/9
CD59 p282 (H19)
CD61 VI-PL2
CD63 H5C6
CD64 10.1
CD69 FN50
CD73 AD2
CD74 LN2
CD79b CB3-1
CD81 5A6
CD82 ASL-24
CD84 CD84.1.21
CD85 MKT5.1
CD85d 42D1
CD85 GHI/75
CD86 IT2.2
CD87 VIM5
CD88 S5/1
CD89 A59
CD93 VIMD2
CD94 DX22
CD95 DX2
CD97 VIM3b
Marker Clone
CD99 HCD99
CD100 A8
CD101 BB27
CD102 CBR-IC2/2
CD116 4H1
CD122 TU27
CD123 6H6
CD124 G077F6
CD126 UV4
CD127 A019D5
CD132 TUGh4
CD134 Ber-ACT35 (ACT35)
CD148 A3
CD154 24-31
CD156c SHM14
CD158a/h HP-MA4
CD158b DX27
CD158e1 DX9
CD161 HP-3G10
CD162 KPL-1
CD163 GHI/61
CD164 67D2
CD165 SN2 (N6-D11)
CD172a SE5A5
CD172b B4B6
CD172g LSB2.20
CD180 MHR73-11
CD182 5E8/CXCR2
CD196 G034E3
CD197 G043H7
CD200 OX-104
CD200R OX-108
CD226 11A8
CD229 Hly-9.1.25
CD244 C1.7
CD268 11C1
CD272 MIH26
CD277 BT3.1
CD278 C398.4A
Marker Clone
CD279 EH12.2H7
CD284 HTA125
CD290 3C10C5
CD298 LNH-94
CD300e UP-H2
CD300F UP-D2
CD314 1D11
CD319 162.1
CD328 6-434
CD335 9E2
CD337 P30-15
CD352 NT-7
CD354 TREM-26
β2-microglobulin 2M2
C3aR hC3aRZ8
CLEC12A 50C1
CX3CR1 2A9-1
FcRL6 2H3
HLA-A,B,C W6/32
HLA-A2 BB7.2
HLA-DQ HLADQ1
HLA-DR L243
HLA-E 3D12
HVEM 122
IgD IA6-2
IgM MHM-88
Integrin β7 FIB504
NKp80 5D12
Siglec-9 K8
TCR gamma/delta B1
TCR Vβ13.2 H132
TCR Vβ23 αHUT7
TCR α/β IP26
TCR Vβ8 JR2 (JR.2)
TCR Vβ9 MKB1
TCR Vδ2 B6
TCR Vγ9 B3
TCR Vα7.2 3C10

100+ Surface Markers Validated on Veri-Cells™ Leukocytes

The following cell surface markers were validated using LEGENDScreen™ Human PE kit.

 

Marker Clone
β2-microglobulin 2M2
CD1c L161
CD1d 51.1
CD2 RPA-2.10
CD3 HIT3a
CD4 RPA-T4
CD5 UCHT2
CD6 BL-CD6
CD10 HI10a
CD11a HI111
CD11b ICRF44
CD13 WM15
CD14 M5E2
CD15 W6D3
CD15 (SSEA-1) MC-480
CD16 3G8
CD18 TS1/18
CD19 HIB19
CD20 2H7
CD21 Bu32
CD22 HIB22
CD23 EBVCS-5
CD24 ML5
CD26 BA5b
CD27 O323
CD28 CD28.2
CD29 TS2/16
CD31 WM59
CD32 Fun-2
CD33 WM53
Marker Clone
CD35 E11
CD38 HIT2
CD39 A1
CD40 HB14
CD43 CD43-10G7
CD44 BJ18
CD45 HI30
CD45RA HI100
CD45RB MEM-55
CD45RO UCHL1
CD47 CC2C6
CD48 BJ40
CD49d 9F10
CD49e NKI-SAM-1
CD50 CBR-IC3/1
CD52 HI186
CD53 HI29
CD55 JS11
CD56 HCD56
CD57 HCD57
CD58 TS2/9
CD59 p282 (H19)
CD64 10.1
CD66a/c/e ASL-32
CD66b G10F5
CD7 CD7-6B7
CD74 LN2
CD81 5A6
CD82 ASL-24
CD84 CD84.1.21
Marker Clone
CD85j GHI/75
CD86 IT2.2
CD87 VIM5
CD88 S5/1
CD89 A59
CD8a HIT8a
CD94 DX22
CD95 DX2
CD97 VIM3b
CD99 HCD99
CD101 BB27
CD102 CBR-IC2/2
CD116 4H1
CD122 TU27
CD127 A019D5
CD132 TUGh4
CD141 M80
CD155 SKII.4
CD156c SHM14
CD161 HP-3G10
CD163 GHI/61
CD164 67D2
CD172a/b SE5A5
CD172b B4B6
CD172g LSB2.20
CD180 MHR73-11
CD197 G043H7
CD200 OX-104
CD226 11A8
CD244 C1.7
Marker Clone
CD245 DY12
CD263 DJR3
CD268 11C1
CD278 C398.4A
CD279 (PD-1) EH12.2H7
CD284 HTA125
CD298 LNH-94
CD300e UP-H2
CD300F UP-D2
CD314 1D11
CD319 162.1
CD328 6-434
CD335 9E2
CD337 P30-15
CD354 TREM-26
CLEC12A 50C1
CX3CR1 2A9-1
DcR1 (TRAIL-R3) DJR3
Galectin-3 (Mac-2) Gal397
HLA-A,B,C W6/32
HLA-DQ HLADQ1
HLA-DR L243
IgD IA6-2
LTβR 31G4D8
NKp80 5D12
Siglec-9 K8
TCR α/β IP26
TCR gamma/delta B1
TCR Vδ2 B6
Vγ9 B3