Where BD Horizon™ V450, Pacific Blue™ and eFluor® 405 are just too dim for fluorescence microscopy, Brilliant Violet 421™ now enables visualization of antigens with directly conjugated antibodies, enhancing your capabilities with multi-color microscopy.
Historically, the "blue" channel on a microscope is reserved for an Hoechst or DAPI counterstaining due to the dim signal of typical dyes in that range, such as Alexa Fluor® 350, coumarin and Pacific Blue™ and the high sample autofluorescence often seen at that short wavelength of excitation. The following figures demonstrate that even on antibodies against markers that are relatively low in abundance, the Brilliant Violet 421™ direct conjugates can be used in imaging applications with high sensitivity. Further, they demonstrate extremely good photostability for repeated imaging and confocal z-stack capture. Also, these direct conjugates of Brilliant Violet 421™ demonstrate that there is no need for secondary antibodies or other amplificatiton techniques in order to achieve sufficient signal. Brilliant Violet 421™ will enable the addition of another color to a multicolor microscopy application on instruments capable of exciting this fluorophore between 360-420 nm.