Brilliant Violet™antibody conjugates will change the future of flow cytometry, bringing new power to the violet laser. In particular, Brilliant Violet 421™ antibodies consistently stain at similar levels to PE, the brightest known fluorochrome, bringing unparalleled sensitivity and resolution to the violet laser, while Brilliant Violet 570™ antibodies add needed versatility to panel selection for multicolor flow cytometry. Brilliant Violet 605™ and Brilliant Violet 650™ antibodies also provide exceptionally bright signal and further expand options for multicolor panels. Brilliant Violet 711™ and Brilliant Violet 785™ bring newly expanded capability and selection for multicolor flow cytometry.

BV421

RBC-lysed human whole blood cells were stained with anti-CD3 conjugated to BV421™, PE, Pacific Blue™ or BD Horizon™ V450, and run on the BD™ LSR II flow cytometer. The stain index values indicated are derived at the optimal concentration for each conjugate. Stain index = (Median Fluorescence Intesity positive cells - Median Fluorescence Instensity negative cells)/2 x rSD.

BV570

RBC-lysed human whole blood cells were stained with anti-CD8 conjugated to BV570™, Pacific Orange™ or BD Horizon™ V500, and run on the BD™ LSR II flow cytometer. The stain index values indicated are derived at the optimal concentration for each conjugate.

BV605

Human whole blood cells were stained with anti-CD8 conjugated to BV605™, or eFluor® 605NC, and run on the BD™ LSR II flow cytometer. The stain index values indicated are derived at the optimal concentration for each conjugate.

BV650

Human whole blood cells were stained with anti-CD8 conjugated to BV650™, or eFluor® 650NC, and run on the BD™ LSR II flow cytometer. The stain index values indicated are derived at the optimal concentration for each conjugate.

Easy to Use and Trouble-Free

Brilliant Violet™ antibodies are simple to use, compatible with standard staining buffers, and stable to fixation. Provided in convenient 5 µl test sizes at optimal ready-to-use concentrations, our Brilliant Violet™ antibody products can easily be added to your multi-color panels. To compare fluorescence spectra data of Brilliant Violet™ conjugates with our other fluorochromes use the BioLegend Fluorescence Spectra Analyzer.

To help with flow panel construction using BioLegend’s Brilliant Violet™ antibodies, use our Multicolor Panel Selector web tool.

Extensive Antibody Selection

BioLegend provides an expansive selection of antibody specificities for Brilliant Violet 421™, Brilliant Violet 570™, Brilliant Violet 605™, Brilliant Violet 650™, Brilliant Violet 711™, and Brilliant Violet 785™. All products are manufactured by our expert chemists in San Diego, CA and are supported by our 100% satisfaction guarantee. For samples or custom conjugations, contact our sales team. 

Brilliant Resolution

cd127

CD127

Human PBMCs were stained with anti-CD3 FITC and anti-CD127 (clone HCD127) conjugated to the BV421™ or Pacific Blue™. The data demonstrates the resolving power of BV421™, clearly showing separation of CD127-positive cells from dim and negative cells which can be difficult to define using Pacific Blue™


 

cd56 image

CD56

Human RBC-lysed whole blood cells were stained in an 11-color panel including HLA-DR PE/Cy7 and anti-CD56 (clone HCD56) conjugated to the BV421™ or anti-CD56 (clone MEM-188) Pacific Blue™. The data reveals discreet resolution of CD56 positive populations with BV421™. Data provided by Axel Schulz / Andreas Thiel, Charité - University Medicine Berlin.


 

tetramer image

Tetramer

Mouse spleen cells were labeled with PBS57-loaded mouse CD1d tetramer bound to Streptavidin-BV421™, -PE, or -Pacific Blue™. The data shows optimal staining with BV421™ when comparing equivalent tetramer concentration at 0.625 µg/ml. Data provided by Dr. Rick Willis, Emory/Yerkes.


 

Brilliant Viability: Nontoxic to Sorted Cells

Brilliant Viability: Nontoxic to Sorted Cells image

Pooled Balb/c spleen and lymph node cells were stained with CD4-BV421™ or CD4-PB and sorted for CD4 positive cells. The cells were then labeled with CFSE and stimulated in 96-well flat-bottom plates coated with anti-CD3/anti-CD28 (1 µg/ml each) at 1 x 105 cells/well. On day 4, cells were analyzed for CFSE dilution. Figure A demonstrates comparable division and expansion of cells sorted by BV421™ compared to those sorted by PB, as indicated by the loss of CFSE signal. Unstimulated cells are displayed as the gray histogram. Figure B shows the median fluorescence values of each group. Error bars represent the standard deviation of triplicate wells. Figure C demonstrates that the % of cells that have divided is comparable between BV421™ and PB-stained cells. Overall, the data shows viability of cells after staining and sorting with a BV421™ antibody. Data provided by Aras Toker/Jochen Hühn, Helmholtz Centre for Infection Research.


 

Brilliant Options with Brilliant Violet 570™

Brilliant Options with Brilliant Violet 570™

Human RBC-lysed whole blood cells were stained with (A) anti-CD8 BV570™ and anti-CD56 BV421™ or (B) anti-CD127 BV570™ and anti-CD25 BV421™. In B, cells were gated on CD4 positive cells. The data demonstrates the capabilities for using BV570™ and BV421™ conjugates together in multicolor panels. Data in B, provided by Eva Tolosa, University Medical Center Hamburg-Eppendorf.

Brilliant Intracellular Detection with Brilliant Violet 570™

image Brilliant Intracellular Detection with Brilliant Violet 570™

PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were surface stained with CD3 FITC and then intracellularly stained with IFN-γ (clone 4S.B3) Brilliant Violet 570™ (right) or mouse IgG1, k Brilliant Violet 570™ isotype control (left).


 

Expanding Brilliance with Brilliant Violet 605™ and Brilliant Violet 650™

 Expanding Brilliance with Brilliant Violet 605™ and Brilliant Violet 650™

Human RBC-lysed whole blood cells were stained with anti-CD8 BV605™ and anti-CD3 BV650™. The data demonstrates distinct positive populations and low background negative populations.


 

Complete Brilliance with Brilliant Violet 711™ and Brilliant Violet 785™

Complete Brilliance with Brilliant Violet 711™ and Brilliant Violet 785™

RBC-lysed human whole blood cells were stained with CD127 BV785™ (clone A019D5) and CD25 BV711™ (clone BC96) to detect Treg cells.