Here at BioLegend, we are impartial when it comes to instrumentation for flow cytometry. Our reagents work beautifully on instruments from all manufacturers for diverse biological applications. But as this field of single cell, fluorescence-based multiplexing continues to grow in total multicolor capacity, sensitivity and ease of use, we can’t wait to release reagents to help match pace with these innovative instruments. 

The Cytek Aurora™ Spectral Cytometer is one of these instruments. The introductory configuration of this instrument is a 3-laser system (405 nm, 488 nm and 633 nm) and provides the possibility for 48 different fluorescent parameters with 2 scatter channels. This is only the theoretical limit as we continue to strive to provide fluorophores that are distinct enough to be accurately unmixed on the system. The Aurora™ also utilizes APD (Avalanche Photodiode) detectors, which liberate the near-IR and infrared wavelengths for more efficient detection of emitted photons in this range.

We will expand on data generated in-house here at BioLegend in this section as we test the limits of spectral flow cytometry, but in the meantime, here is a glimpse of 21 color data from 3 lasers with the use of fluorophores that spectrally overlap almost identically on traditional machines. For example, in the panel to the right, PerCP and PerCP/Cy5.5, Alexa Fluor® 647 and APC, Brilliant Violet 421™ and Pacific Blue™ are all utilized at the same time. It also allows researchers to use eight Brilliant Violet™ (BV421™, BV510™, BV570™, BV605™, BV650™, BV711™, the new BV750™, and BV785™) fluorophores together with minimal compensation concerns. If you have additional questions on this panel, feel free to contact technical service

(Right) Human whole blood was stained with 21 different antibody conjugates and analyzed on the Cytek Aurora™ Spectral Flow Cytometer.

Specificity Format
CD303 PE/Cy7
CD16 BV570™
CD14 PerCP/Cy5.5
CD1c BV711™
IgD BV510™
CD20 APC/Fire™ 750
CD8 Pacific Blue™
CD3 Alex Fluor® 700
CD25 BV421™
CCR6 BV785™
CD56 BV750™
CD27 PerCP
CD127 PE/Cy5
CD38 PE/Dazzle™ 594
HLA-DR Alexa Fluor® 647
CD197 BV650™
CCR4 BV605™

T Cell Analysis

B Cell Analysis

Monocyte and Dendritic Cell Analysis