BioLegend Flex-T
BioLegend is happy to introduce our new family of reagents to study antigen-specific T cells. Flex-T™, Major Histocompatibility Complex (MHC) tetramers, brings to you the flexibility of loading any peptide of interest into the binding site of the complex, by using ultraviolet light labile peptides. The efficiency of peptide exchange can be monitored through a simple ELISA assay. At the moment we offer individual components for the ELISA assay, please follow the protocol, found in the "Products and More" tab.
Introduction Combinatorial Color Coding Representative Data Products and More FAQs
Introduction
What are MHC tetramers and what can you do with them?
The T-cell mediated immune response is defined by the interaction between antigen presenting cells and T cells, through the Major Histocompatibility Complex (MHC) and the T cell receptor (TCR). MHC molecules present a peptide to antigen-specific T cells that recognize this peptide. Soluble, monomeric MHC molecules bind very weakly to the TCR. However, by making a tetramer through a fluorescently labeled streptavidin conjugate, the complex has greater avidity to the T cell and maintains more stable binding by interacting with several TCRs, making it useful for flow cytometry detection of antigen specific T cells.
What is a Flex-T™?
Flex-T™ is BioLegend's technology to study antigen-specific T cells through their TCRs. It has the unique property of allowing the loading of peptides of interest into the binding site of the MHC groove, by using ultraviolet (UV) light labile, exchangeable peptides. Flex-T™ technology features:
  • Flexible peptide loading
  • Two color combination capability
  • High throughput screening
  • High specificity
  • Ease of use
  • Affordability
How does ultraviolet peptide exchange work?
Flex-T™ is made of MHC monomers loaded with a peptide that can be degraded by the use of a UV light source. This allows for a peptide exchange when the UV irradiation is done in the presence of the peptide of interest (which is not UV-labile). This flexibility permits the screening of virtually any peptide of interest with enough affinity for the MHC allele that it is loaded onto.
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