Product: APC anti-mouse CD93 (AA4.1, early B lineage) |
Catalog No.: 136510 |
Adam Coleman, Postdoctoral Fellow Oak Ridge Institute for Science and Education |
Reliable Results (APC anti-mouse CD93 (AA4.1, early B lineage)) | |||
Overall: | Product Quality: | Ease of Use: | |
Good color/antibody for early B cell lineage work. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | 5 day old mouse splenocytes |
Brief Protocol: | Mash spleen through nylon mesh Purify mouse B cells from spleen using magnetic separation column Wash cells in FACS buffer (1x PBS + 2% FBS + 2mM EDTA) Resuspend cells in 100 ul FACS buffer + 1 ul Alexa Fluor 700 anti-mouse CD45R/B220, + 1 ul APC anti-mouse CD93 on ice for 10 minutes Wash cells in FACS buffer and analyze on flow cytometer |
Results Summary: | Neonatal spleens contain CD93+ immature B cells |
Additional Notes: |
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