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Products
Antibodies
Primary Antibodies
To detect your protein of interest, we provide highly specific primary antibodies validated and quality-tested by western blotting. Our antibodies cover a wide range of targets across cell biology, neuroscience, immunology, and other key signaling pathways. These come in either a purified, unconjugated format or directly conjugated to HRP.
Find Western Blotting Primary Antibodies
Secondary Reagents
Secondary reagents target the primary antibody and are conjugated to provide visualization of the protein of interest. For western blotting, these reagents are conjugated to HRP which is used as a reporter enzyme.
Knockout/Knockdown Validation
As knocking out the target protein is one of the most trusted antibody validation processes, we validate many of our cell biology antibodies by KO (knockout) and KD (knockdown) systems.
The reproducibility of published research has emerged as an urgent topic in today’s scientific community. From funding agencies to researchers to manufacturers and publishers, it is critical for all of these groups to align themselves to ensure that research is done with rigor and is reproducible.
Total lysates from HeLa and HeLa STAT3 CRISPR/Cas9 knockout (KO) cells were probed with 1 µg/ml purified anti-STAT3 antibody. Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower).
PhosphoPair Antibody Set
Try PhosphoPair Antibody Sets for a convenient, cost-saving solution to interrogate phosphorylation and cell signaling pathways. Each set bundles an antibody specific for a phosphorylated protein with one that recognizes total protein. Sets are available for critical signaling mediators like STAT3, ERK, and mTOR.
Key Features:
- Each antibody is quality-tested by western blot.
- Supplied in a purified format at 25 µg per vial.
Western blot analysis using whole cell extracts from serum starved HeLa and NIH/3T3 cells treated with rapamycin (Rap) or insulin (Ins). Membranes were probed with purified anti-mTOR Phospho (Ser2448) and a duplicate membrane was pre-treated with lambda protein phosphatase prior to incubation. Equal mTOR loading was confirmed by probing membranes with an anti-pan mTOR antibody.
Antibody Sampler Kits
Antibody sampler kits provide a convenient and economical resource to test multiple antibodies. Each kit contains at least 4 antibodies kits and target cell function and structure proteins, related detection methods (such as epitope tags), or proteins connected through signaling pathways.
Protein Ladders
Our Prime-Step™ Prestained Broad Range Protein Ladder is a three-color protein standard with 10 prestained chromophore-conjugated recombinant proteins covering a wide range of molecular weights—from 6.5 to 270 kDa. It is ideal for monitoring protein separation during SDS-Page, confirming WB transfer on membranes, and estimating protein sizes.
3 µL Prime-Step™ Prestained Broad Range Protein Ladder was resolved by electrophoresis in different gel running systems, including 14%, 4-20% and 4-12% Tris-Glycine gel, 4-12% Bis-Tris gel (MOPS and MES running buffer), 3-8% Tris-Acetate gel and 10-20% Tricine gel. Protein ladders were transferred to a nitrocellulose membrane.
ECL Substrates
Our Western-Ready™ ECL Substrate Kits are a non-radioactive enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) activity from WB reagents and ELISA immunoassays. Our kits provide sensitive detection and sustained signal for up to 4 hours. Our ECL Plus Kit is ideal for detecting low- to mid- picogram levels of antigen, while the Premium Kit provides more sensitive detection of high-femtogram levels of protein.
Key features:
- Luminol-based detection system
- Sensitive detection
- Available in small 20 mL size for sampling
- Multiple kits with a range of sensitivities
The indicated amounts of whole cell extract from HeLa cells were probed with purified anti-Lamin A/C antibody. Proteins were visualized using HRP goat anti-mouse IgG antibody, followed by incubation with Western-Ready™ ECL Substrate Plus Kit or Western-Ready™ ECL Substrate Premium Kit.
Western-Ready™ Buffers
Try our complete set of Western-Ready™ buffers for every step of your workflow, including protein extraction, gel loading and running, and detection.
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Description |
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Running buffer for resolving small-to-medium-sized proteins on Bis-Tris SDS page gels. |
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Used for the preparation of samples for non-reducing and reducing SDS-PAGE. |
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Stringent lysis buffer to extract proteins from all subcellular compartments. |
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Buffer for blocking, washing, and antibody dilution. Formulated to reduce non-specific binding. |
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| Western-Ready™ Transfer Buffer (10X) | Buffer formulated for western blot protein transfer onto PVDF or nitrocellulose membranes. |
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