Antibody-oligo conjugates are enabling a new era of research offering unprecedented insights into cellular processes. Single-cell multiomics allows for simultaneous profiling of RNA and protein from a single cell. CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) and similar methods, seamlessly integrate antibody-oligo conjugates with existing single-cell sequencing workflows, allowing antibody-directed quantification of cellular proteins in parallel with the RNA transcriptome.

BioLegend’s revolutionary TotalSeq™ oligo-conjugated antibodies provide a standardized process for antibody-directed protein characterization. This allows for measurement of cell surface proteins without the limitations of fluorophores or heavy metals. To date, we have successfully multiplexed more than 220 proteins. TotalSeq™ also has Cell Hashing antibodies to facilitate sample multiplexing by targeting ubiquitously expressed surface proteins. TotalSeq™ Cell Hashing antibodies combined with antibody directed proteomics enable a stream-lined process for combining multiple sample sources incorporating targets from both the proteome and transcriptome of single cells.

Join us to review the use of TotalSeq™ antibodies in exciting applications such as CITE-seq, and how it interfaces with commercial scRNAseq solutions such as the 10X Genomics system with Feature Barcoding Technology™. We will discuss technical considerations, collaborator data, new products in development, and more.

BioLegend Seminar: TotalSeq™, Revolutionary Highly Multiplexed Protein Analysis in Single-Cell Multi-Omics

Date: June 2^nd 2020 at 12 pm CST

Speaker: Sanna Younger, Ph.D., Technical Applications Scientist, BioLegend Inc.